Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse NEDD4-binding protein 1 (N4BP1) Standard Curve.)
Mouse NEDD4-Binding Protein 1 (N4BP1) ELISA Kit | N4BP1 elisa kit
Mouse NEDD4-Binding Protein 1 (N4BP1) ELISA Kit
Gene Names
N4bp1; C81621; AI481586
Reactivity
Mouse
Synonyms
NEDD4-Binding Protein 1 (N4BP1); Mouse NEDD4-Binding Protein 1 (N4BP1) ELISA Kit; N4BP1; FLJ31821; KIAA0615; MGC176730; Nedd4 binding protein 1; N4BP1 elisa kit
Reactivity
Mouse
Specificity
Mouse NEDD4-binding protein 1 (N4BP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse N4BP1. No significant cross-reactivity or interference between Mouse N4BP1 and analogues was observed.
Sequence Length
893
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse NEDD4-binding protein 1 (N4BP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse NEDD4-binding protein 1 (N4BP1) Standard Curve.)
Related Product Information for N4BP1 elisa kit
Description: This Mouse NEDD4-binding protein 1 (N4BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate N4BP1 in samples. An antibody specific for N4BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any N4BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for N4BP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of N4BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: N4BP1 (NEDD4 Binding Protein 1) is a Protein Coding gene. GO annotations related to this gene include RNA binding. An important paralog of this gene is KHNYN.
Background: N4BP1 (NEDD4 Binding Protein 1) is a Protein Coding gene. GO annotations related to this gene include RNA binding. An important paralog of this gene is KHNYN.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
NEDD4-binding protein 1
NCBI Official Synonym Full Names
NEDD4 binding protein 1
NCBI Official Symbol
N4bp1
NCBI Official Synonym Symbols
C81621; AI481586
NCBI Protein Information
NEDD4-binding protein 1
UniProt Protein Name
NEDD4-binding protein 1
Protein Family
UniProt Gene Name
N4bp1
UniProt Synonym Gene Names
Kiaa0615; N4BP1
UniProt Entry Name
N4BP1_MOUSE
Uniprot Description
N4BP1: Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. Belongs to the N4BP1 family.
Protein type: Nucleolus; Unknown function
Cellular Component: PML body; nucleolus; nucleus
Molecular Function: protein binding; RNA binding
Biological Process: negative regulation of protein ubiquitination; negative regulation of proteasomal ubiquitin-dependent protein catabolic process
Research Articles on N4BP1
Similar Products
Product Notes
The Mouse N4BP1 n4bp1 (Catalog #AAA9717456) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717456 ELISA Kit recognizes Mouse N4BP1. It is sometimes possible for the material contained within the vial of "NEDD4-Binding Protein 1 (N4BP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
