Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Neural cell adhesion molecule 1 (NCAM1) Standard Curve.)
Mouse Neural Cell Adhesion Molecule 1 (NCAM1) ELISA Kit | NCAM1 elisa kit
Mouse Neural Cell Adhesion Molecule 1 (NCAM1) ELISA Kit
Gene Names
Ncam1; CD56; Ncam; E-NCAM; NCAM-1
Reactivity
Mouse
Synonyms
Neural Cell Adhesion Molecule 1 (NCAM1); Mouse Neural Cell Adhesion Molecule 1 (NCAM1) ELISA Kit; NCAM1; CD56; MSK39; NCAM; antigen recognized by monoclonal antibody 5.1H11; neural cell adhesion molecule; NCAM1 elisa kit
Reactivity
Mouse
Specificity
Mouse Neural cell adhesion molecule 1 (NCAM1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NCAM1. No significant cross-reactivity or interference between Mouse NCAM1 and analogues was observed.
Sequence Length
1115
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Neural cell adhesion molecule 1 (NCAM1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Neural cell adhesion molecule 1 (NCAM1) Standard Curve.)
Related Product Information for NCAM1 elisa kit
Description: This Mouse Neural cell adhesion molecule 1 (NCAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate NCAM1 in samples. An antibody specific for NCAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NCAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NCAM1 encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The Neural Cell Adhesion Molecule 1 is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.
Background: NCAM1 encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The Neural Cell Adhesion Molecule 1 is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Neural cell adhesion molecule 1
NCBI Official Synonym Full Names
neural cell adhesion molecule 1
NCBI Official Symbol
Ncam1
NCBI Official Synonym Symbols
CD56; Ncam; E-NCAM; NCAM-1
NCBI Protein Information
neural cell adhesion molecule 1
UniProt Protein Name
Neural cell adhesion molecule 1
Protein Family
UniProt Gene Name
Ncam1
UniProt Synonym Gene Names
Ncam; N-CAM-1; NCAM-1
UniProt Entry Name
NCAM1_MOUSE
Uniprot Description
NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, GPI anchor; Motility/polarity/chemotaxis
Cellular Component: cell surface; growth cone; membrane; cell soma; axon; integral to membrane; plasma membrane; intercellular junction; myelin sheath; external side of plasma membrane
Molecular Function: heparin binding; identical protein binding; protein binding; LRR domain binding; phosphatase binding
Biological Process: regulation of exocyst assembly; positive regulation of calcium-mediated signaling; thalamus development; response to cocaine; cell surface receptor linked signal transduction; learning and/or memory; homotypic cell-cell adhesion; response to lead ion; response to fluoxetine; neuron development; regulation of sensory perception of pain; cell adhesion; multicellular organismal response to stress; neurite development; aging; positive regulation of cardiac muscle cell proliferation
Research Articles on NCAM1
Similar Products
Product Notes
The Mouse NCAM1 ncam1 (Catalog #AAA9717417) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717417 ELISA Kit recognizes Mouse NCAM1. It is sometimes possible for the material contained within the vial of "Neural Cell Adhesion Molecule 1 (NCAM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
