Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pregnancy associated plasma protein A (PAPP-A) Standard Curve.)
Mouse Pregnancy Associated Plasma Protein A (PAPP-A) ELISA Kit | PAPP-A elisa kit
Mouse Pregnancy Associated Plasma Protein A (PAPP-A) ELISA Kit
Gene Names
Pappa; PAG1; PAPP-A; IGFBP-4ase; 8430414N03Rik
Reactivity
Mouse
Synonyms
Pregnancy Associated Plasma Protein A (PAPP-A); Mouse Pregnancy Associated Plasma Protein A (PAPP-A) ELISA Kit; PAPP-A; RP11-45A16.1; ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPPA1; aspecific BCL2 ARE-binding protein 2; differentially placenta 1 expressed protein; insulin-like growth factor-dependent IGF binding pro; PAPP-A elisa kit
Reactivity
Mouse
Specificity
Mouse Pregnancy associated plasma protein A (PAPP-A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PAPP-A. No significant cross-reactivity or interference between Mouse PAPP-A and analogues was observed.
Sequence Length
1624
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pregnancy associated plasma protein A (PAPP-A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pregnancy associated plasma protein A (PAPP-A) Standard Curve.)
Related Product Information for PAPP-A elisa kit
Description: This Mouse Pregnancy associated plasma protein A (PAPP-A) ELISA Kit employs a two-site sandwich ELISA to quantitate PAPP-A in samples. An antibody specific for PAPP-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAPP-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAPP-A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAPP-A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PAPP-A is a large heterottetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down's syndrome. a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses (fetuses with an abnormal number of chromosomes). For example, low PAPPA may be seen in prenatal screening for Down syndrome.
Background: PAPP-A is a large heterottetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down's syndrome. a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses (fetuses with an abnormal number of chromosomes). For example, low PAPPA may be seen in prenatal screening for Down syndrome.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pappalysin-1
NCBI Official Synonym Full Names
pregnancy-associated plasma protein A
NCBI Official Symbol
Pappa
NCBI Official Synonym Symbols
PAG1; PAPP-A; IGFBP-4ase; 8430414N03Rik
NCBI Protein Information
pappalysin-1
UniProt Protein Name
Pappalysin-1
UniProt Gene Name
Pappa
UniProt Synonym Gene Names
IGF-dependent IGFBP-4 protease; IGFBP-4ase; PAPP-A
Uniprot Description
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Isoform 2 cleaves IGFBP-4 very slowly compared to PAPP-A, but its ability to cleave IGFBP-5 is unaffected.
Research Articles on PAPP-A
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Product Notes
The Mouse PAPP-A pappa (Catalog #AAA9717398) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717398 ELISA Kit recognizes Mouse PAPP-A. It is sometimes possible for the material contained within the vial of "Pregnancy Associated Plasma Protein A (PAPP-A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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