Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble platelet endothelial cell adhesion molecule 1 (sPECAM-1) Standard Curve.)
Mouse Soluble Platelet Endothelial Cell Adhesion Molecule 1 (sPECAM-1) ELISA Kit | sPECAM-1 elisa kit
Mouse Soluble Platelet Endothelial Cell Adhesion Molecule 1 (sPECAM-1) ELISA Kit
Gene Names
Pecam1; Cd31; Pecam; C85791; PECAM-1
Reactivity
Mouse
Synonyms
Soluble Platelet Endothelial Cell Adhesion Molecule 1 (sPECAM-1); Mouse Soluble Platelet Endothelial Cell Adhesion Molecule 1 (sPECAM-1) ELISA Kit; PECAM1; CD31; FLJ58394; PECAM-1; CD31 antigen; CD31/EndoCAM; adhesion molecule; sPECAM-1 elisa kit
Reactivity
Mouse
Specificity
Mouse Soluble platelet endothelial cell adhesion molecule 1 (sPECAM-1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PECAM1. No significant cross-reactivity or interference between Mouse PECAM1 and analogues was observed.
Sequence Length
727
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble platelet endothelial cell adhesion molecule 1 (sPECAM-1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble platelet endothelial cell adhesion molecule 1 (sPECAM-1) Standard Curve.)
Related Product Information for sPECAM-1 elisa kit
Description: This Mouse Soluble platelet endothelial cell adhesion molecule 1 (sPECAM-1) ELISA Kit employs a two-site sandwich ELISA to quantitate PECAM1 in samples. An antibody specific for PECAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PECAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PECAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PECAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The platelet/endothelial cell adhesion molecule 1 encoded by Pecam1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Background: The platelet/endothelial cell adhesion molecule 1 encoded by Pecam1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Platelet endothelial cell adhesion molecule
NCBI Official Synonym Full Names
platelet/endothelial cell adhesion molecule 1
NCBI Official Symbol
Pecam1
NCBI Official Synonym Symbols
Cd31; Pecam; C85791; PECAM-1
NCBI Protein Information
platelet endothelial cell adhesion molecule
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Gene Name
Pecam1
UniProt Synonym Gene Names
Pecam; Pecam-1; PECAM-1
UniProt Entry Name
PECA1_MOUSE
Uniprot Description
PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.
Protein type: Cell adhesion; Membrane protein, integral
Cellular Component: extracellular space; cell surface; smooth muscle contractile fiber; membrane; integral to membrane; plasma membrane; intracellular; cell junction; lipid raft; external side of plasma membrane
Molecular Function: protein binding
Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; wound healing; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of actin filament polymerization; positive regulation of leukocyte migration; diapedesis; cell adhesion; regulation of cell migration; phagocytosis; Rho protein signal transduction
Research Articles on sPECAM-1
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Product Notes
The Mouse sPECAM-1 pecam1 (Catalog #AAA9717381) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717381 ELISA Kit recognizes Mouse sPECAM-1. It is sometimes possible for the material contained within the vial of "Soluble Platelet Endothelial Cell Adhesion Molecule 1 (sPECAM-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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