Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serine/threonine-protein kinase pim-2 (PIM2) Standard Curve.)
Mouse Serine/Threonine-Protein Kinase pim-2 (PIM2) ELISA Kit | PIM2 elisa kit
Mouse Serine/Threonine-Protein Kinase pim-2 (PIM2) ELISA Kit
Gene Names
Pim2; Pim-2; DXCch3
Reactivity
Mouse
Synonyms
Serine/Threonine-Protein Kinase pim-2 (PIM2); Mouse Serine/Threonine-Protein Kinase pim-2 (PIM2) ELISA Kit; PIM2; Proto-oncogene Pim-2 (serine threonine kinase); serine/threonine protein kinase pim-2; PIM2 elisa kit
Reactivity
Mouse
Specificity
Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIM2. No significant cross-reactivity or interference between Mouse PIM2 and analogues was observed.
Sequence Length
370
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serine/threonine-protein kinase pim-2 (PIM2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serine/threonine-protein kinase pim-2 (PIM2) Standard Curve.)
Related Product Information for PIM2 elisa kit
Description: This Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PIM2 in samples. An antibody specific for PIM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PIM2 encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival. The deduced 334-amino acid sequence of the clone showed 90% identity with the mouse Pim2 protein. Like mouse Pim2, the human protein appears to be a serine threonine kinase. Northern blot analysis detected 2 PIM2 transcripts. A 2.2-kb transcript was expressed in all tissues tested, but most abundantly in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It was also highly expressed in human leukemic and lymphoma cell lines and a colorectal adenocarcinoma cell line. A 5.0-kb transcript was expressed only in spleen, thymus, small intestine, colon, and in very small amounts in peripheral blood leukocytes.
Background: PIM2 encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival. The deduced 334-amino acid sequence of the clone showed 90% identity with the mouse Pim2 protein. Like mouse Pim2, the human protein appears to be a serine threonine kinase. Northern blot analysis detected 2 PIM2 transcripts. A 2.2-kb transcript was expressed in all tissues tested, but most abundantly in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It was also highly expressed in human leukemic and lymphoma cell lines and a colorectal adenocarcinoma cell line. A 5.0-kb transcript was expressed only in spleen, thymus, small intestine, colon, and in very small amounts in peripheral blood leukocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine/threonine-protein kinase pim-2
NCBI Official Synonym Full Names
proviral integration site 2
NCBI Official Symbol
Pim2
NCBI Official Synonym Symbols
Pim-2; DXCch3
NCBI Protein Information
serine/threonine-protein kinase pim-2
UniProt Protein Name
Serine/threonine-protein kinase pim-2
Protein Family
UniProt Gene Name
Pim2
UniProt Synonym Gene Names
Pim-2
UniProt Entry Name
PIM2_MOUSE
Research Articles on PIM2
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Product Notes
The Mouse PIM2 pim2 (Catalog #AAA9717357) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717357 ELISA Kit recognizes Mouse PIM2. It is sometimes possible for the material contained within the vial of "Serine/Threonine-Protein Kinase pim-2 (PIM2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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