Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pituitary homeobox 2 (PITX2) Standard Curve.)
Mouse Pituitary Homeobox 2 (PITX2) ELISA Kit | PITX2 elisa kit
Mouse Pituitary Homeobox 2 (PITX2) ELISA Kit
Gene Names
Pitx2; Brx1; Ptx2; Rieg; Brx1b; Otlx2; Munc30; 9430085M16Rik
Reactivity
Mouse
Synonyms
Pituitary Homeobox 2 (PITX2); Mouse Pituitary Homeobox 2 (PITX2) ELISA Kit; PITX2; ARP1; Brx1; IDG2; IGDS; IGDS2; IHG2; IRID2; MGC111022; MGC20144; Otlx2; PTX2; RGS; RIEG; RIEG1; RS; OTTHUMP00000163736; all1-responsive gene 1; paired-like homeodomain transcription factor 2; pituitary; PITX2 elisa kit
Reactivity
Mouse
Specificity
Mouse Pituitary homeobox 2 (PITX2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PITX2. No significant cross-reactivity or interference between Mouse PITX2 and analogues was observed.
Sequence Length
324
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pituitary homeobox 2 (PITX2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pituitary homeobox 2 (PITX2) Standard Curve.)
Related Product Information for PITX2 elisa kit
Description: This Mouse Pituitary homeobox 2 (PITX2) ELISA Kit employs a two-site sandwich ELISA to quantitate PITX2 in samples. An antibody specific for PITX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PITX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PITX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PITX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PITX2 encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. Mutations in this gene are associated with Axenfeld-Rieger syndrome (ARS), iridogoniodysgenesis syndrome (IGDS), and sporadic cases of Peters anomaly. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes. This protein is involved in the development of the eye, tooth and abdominal organs. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development.
Background: PITX2 encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. Mutations in this gene are associated with Axenfeld-Rieger syndrome (ARS), iridogoniodysgenesis syndrome (IGDS), and sporadic cases of Peters anomaly. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes. This protein is involved in the development of the eye, tooth and abdominal organs. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pituitary homeobox 2 isoform c
NCBI Official Synonym Full Names
paired-like homeodomain transcription factor 2
NCBI Official Symbol
Pitx2
NCBI Official Synonym Symbols
Brx1; Ptx2; Rieg; Brx1b; Otlx2; Munc30; 9430085M16Rik
NCBI Protein Information
pituitary homeobox 2
UniProt Protein Name
Pituitary homeobox 2
Protein Family
UniProt Gene Name
Pitx2
UniProt Synonym Gene Names
Arp1; Brx1; Otlx2; Ptx2; Rgs
UniProt Entry Name
PITX2_MOUSE
Research Articles on PITX2
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Product Notes
The Mouse PITX2 pitx2 (Catalog #AAA9717353) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717353 ELISA Kit recognizes Mouse PITX2. It is sometimes possible for the material contained within the vial of "Pituitary Homeobox 2 (PITX2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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