Mouse ATP-Binding Cassette Sub-Family G Member 2 (ABCG2) ELISA Kit | ABCG2 elisa kit

Mouse ATP-Binding Cassette Sub-Family G Member 2 (ABCG2) ELISA Kit

Cat. Num. AAA9717140

• Gene Names: Abcg2; MXR; ABCP; BCRP; MXR1; ABC15; Bcrp1; AI428558
• Reactivity: Mouse
• Synonyms: ATP-Binding Cassette Sub-Family G Member 2 (ABCG2); Mouse ATP-Binding Cassette Sub-Family G Member 2 (ABCG2) ELISA Kit; ABCG2; ABC15; ABCP; BCRP; BCRP1; BMDP; CD338; CDw338; EST157481; MGC102821; MRX; MXR; MXR1; ABC transporter; ATP-binding cassette transporter G2; ATP-binding cassette; sub-family G; member 2; breast cancer re; ABCG2 elisa kit

• Reactivity: Mouse
• Specificity: Mouse ATP-binding cassette sub-family G member 2 (ABCG2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ABCG2. No significant cross-reactivity or interference between Mouse ABCG2 and analogues was observed.
• Sequence Length: 657

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse ATP-binding cassette sub-family G member 2 (ABCG2) Standard Curve.)

Related Product Information for ABCG2 elisa kit

Description: This Mouse ATP-binding cassette sub-family G member 2 (ABCG2) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCG2 in samples. An antibody specific for ABCG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABCG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: ATP-binding cassette, sub-family G (WHITE), member 2 is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.

NCBI and Uniprot Product Information

• NCBI GI #: 68052328
• NCBI GeneID: 26357
• UniProt Accession #: Q7TMS5
• NCBI Official Full Name: ATP-binding cassette sub-family G member 2
• NCBI Official Synonym Full Names: ATP binding cassette subfamily G member 2 (Junior blood group)
• NCBI Official Symbol: Abcg2
• NCBI Official Synonym Symbols: MXR; ABCP; BCRP; MXR1; ABC15; Bcrp1; AI428558
• NCBI Protein Information: ATP-binding cassette sub-family G member 2
• UniProt Protein Name: ATP-binding cassette sub-family G member 2
• Protein Family: ABC transporter G family
• UniProt Gene Name: Abcg2
• UniProt Synonym Gene Names: Abcp; Bcrp1
• UniProt Entry Name: ABCG2_MOUSE

NCBI Description

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, the human protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. This protein likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. [provided by RefSeq, Jul 2008]