Western Blot (WB)
(All lanes : Anti-DVL3 Antibody (C-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLane 3: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 78 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit DVL3 Polyclonal Antibody | anti-DVL3 antibody
DVL3 Antibody (C-term)
Western Blot (WB)
(All lanes : Anti-DVL3 Antibody (C-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLane 3: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 78 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-DVL3 Antibody (C-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLane 3: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 78 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(DVL3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DVL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DVL3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DVL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DVL3 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DVL3 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
strong similarity with the Drosophila dishevelled gene, dsh. The
Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein
that regulates cell proliferation.
Bouteille, N., et al. Oncogene 28(28):2569-2580(2009)
Fukukawa, C., et al. Oncogene 28(8):1110-1120(2009)
Wei, Q., et al. Lung Cancer 62(2):181-192(2008)
Lee, Y.N., et al. Cell. Signal. 20(2):443-452(2008)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq, Jul 2008]
Uniprot Description
DVL3: a protein in the WNT/planar cell polarity (PCP) signaling pathway. Dvl2-deficient mice indicate that Dvl2 is essential for normal cardiac morphogenesis, somite segmentation and neural tube closure. Transduces the Wnt signal by interacting with the cytoplasmic Axin complex. Dvl and Axin each contain a DIX domains, which mediate their dynamic polymerization. The Dvl-Axin interaction is essential for Wnt signaling. Interacts through its PDZ domain with the C-terminal regions of VANGL1 and VANGL2. Interacts with Dixin and Rac. There is functional redundancy between Dvl1 and Dvl2 in some phenotypes.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 3q27
Cellular Component: cytosol
Molecular Function: protein binding; protease binding; protein heterodimerization activity; frizzled binding; beta-catenin binding; receptor binding
Biological Process: response to drug; positive regulation of JNK activity; Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation
Research Articles on DVL3
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Product Notes
The DVL3 dvl3 (Catalog #AAA9211093) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 530-557. The DVL3 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's DVL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). WB~~1:2000. Researchers should empirically determine the suitability of the DVL3 dvl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DVL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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