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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Vasoactive Intestinal Peptide (VIP) Standard Curve.)

Mouse Vasoactive Intestinal Peptide (VIP) ELISA Kit | VIP elisa kit

Mouse Vasoactive Intestinal Peptide (VIP) ELISA Kit

Reactivity
Mouse
Synonyms
Vasoactive Intestinal Peptide (VIP); Mouse Vasoactive Intestinal Peptide (VIP) ELISA Kit; VIP; MGC13587; PHM27; VIP elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Mouse Vasoactive Intestinal Peptide (VIP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse VIP. No significant cross-reactivity or interference between Mouse VIP and analogues was observed.
Sequence Length
170
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Vasoactive Intestinal Peptide (VIP) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Vasoactive Intestinal Peptide (VIP) Standard Curve.)
Related Product Information for VIP elisa kit
Description: This Mouse Vasoactive Intestinal Peptide (VIP) ELISA Kit employs a two-site sandwich ELISA to quantitate VIP in samples. An antibody specific for VIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Vasoactive intestinal peptide (VIP) is a peptide hormone containing 28 amino acid residues and is produced in many areas of the human body including the gut, pancreas and suprachiasmatic nuclei of the hypothalamus in the brain.It has a half-life in the blood of about two minutes. With respect to the digestive system, VIP seems to induce smooth muscle relaxation (lower esophageal sphincter, stomach, gallbladder), stimulate secretion of water into pancreatic juice and bile, and cause inhibition of gastric acid secretion and absorption from the intestinal lumen. Its role in the intestine is to greatly stimulate secretion of water and electrolytes, as well as dilating intestinal smooth muscle, dilating peripheral blood vessels, stimulating pancreatic bicarbonate secretion, and inhibiting gastrin-stimulated gastric acid secretion.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
VIP peptides isoform 2 preproprotein
NCBI Official Synonym Full Names
vasoactive intestinal polypeptide
NCBI Official Symbol
Vip
NCBI Protein Information
VIP peptides
UniProt Protein Name
VIP peptides
Protein Family
UniProt Gene Name
Vip
UniProt Synonym Gene Names
VIP

NCBI Description

This gene encodes a neuropeptide of the glucagon/secretin superfamily with potent bronchodilator, immunomodulator and anti-inflammatory properties. The encoded protein is proteolytically processed to generate two structurally similar neuropeptides - vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI). In the digestive tract, VIP stimulates relaxation of enteric smooth muscle, secretion of water and electrolytes, release of insulin and glucagon, and inhibition of gastric acid secretion. In the cardiovascular system, VIP causes coronary vasodilation and stimulates contractility in the heart. Mice lacking VIP exhibit airway hyperresponsiveness and airway inflammation. Male mice lacking VIP exhibit moderate pulmonary arterial hypertension resulting in increased mortality. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin (). PHM also causes vasodilation.

Research Articles on VIP

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Product Notes

The Mouse VIP vip (Catalog #AAA9716631) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716631 ELISA Kit recognizes Mouse VIP. It is sometimes possible for the material contained within the vial of "Vasoactive Intestinal Peptide (VIP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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