Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Hydroxylysine glycosides (HOLG) Standard Curve.)
Human Hydroxylysine Glycosides (HOLG) ELISA Kit | HOLG elisa kit
Human Hydroxylysine Glycosides (HOLG) ELISA Kit
Reactivity
Human
Synonyms
Hydroxylysine Glycosides (HOLG); Human Hydroxylysine Glycosides (HOLG) ELISA Kit; HOLG elisa kit
Reactivity
Human
Specificity
Human Hydroxylysine glycosides (HOLG) ELISA Kit has high sensitivity and excellent specificity for detection of Human HOLG. No significant cross-reactivity or interference between Human HOLG and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Hydroxylysine glycosides (HOLG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Hydroxylysine glycosides (HOLG) Standard Curve.)
Related Product Information for HOLG elisa kit
Description: This Human Hydroxylysine glycosides (HOLG) ELISA Kit employs a two-site sandwich ELISA to quantitate HOLG in samples. An antibody specific for HOLG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HOLG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HOLG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HOLG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Senile osteoporosis (SOP), also known as degenerative osteoporosis, a special performance of biological aging in bone are the occurrence of osteoporosis type II, it is growing with age, bone mineral composition and reducing the proportion of bone matrix. Bone thinning, the reduction in the number of bone small beam. A systemic metabolic disorder of increased bone fragility and risk of fracture increased degenerative diseases. It is the increase in bone fragility and lead to fracture increases the risk of a common disease. Women generally in postmenopausal more than 20 years. Male age around 70 years of age or more, the incidence of female-male 2 times. Type trabecular and cortical bone, bone loss with age-related bone loss. Began 40 to 50-year-old men and women with age-related bone loss in the spine, gender rate of bone loss similar to the limbs lost 0.3% to 0.6% annually, spine lost 0.8% to 1.2% annually. Fractures occur in the spine and hip.
Background: Senile osteoporosis (SOP), also known as degenerative osteoporosis, a special performance of biological aging in bone are the occurrence of osteoporosis type II, it is growing with age, bone mineral composition and reducing the proportion of bone matrix. Bone thinning, the reduction in the number of bone small beam. A systemic metabolic disorder of increased bone fragility and risk of fracture increased degenerative diseases. It is the increase in bone fragility and lead to fracture increases the risk of a common disease. Women generally in postmenopausal more than 20 years. Male age around 70 years of age or more, the incidence of female-male 2 times. Type trabecular and cortical bone, bone loss with age-related bone loss. Began 40 to 50-year-old men and women with age-related bone loss in the spine, gender rate of bone loss similar to the limbs lost 0.3% to 0.6% annually, spine lost 0.8% to 1.2% annually. Fractures occur in the spine and hip.
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Product Notes
The Human HOLG (Catalog #AAA9716185) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716185 ELISA Kit recognizes Human HOLG. It is sometimes possible for the material contained within the vial of "Hydroxylysine Glycosides (HOLG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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