Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Integral membrane protein GPR137B (GPR137B) Standard Curve.)
Human Integral Membrane Protein GPR137B (GPR137B) ELISA Kit | GPR137B elisa kit
Human Integral Membrane Protein GPR137B (GPR137B) ELISA Kit
Gene Names
GPR137B; TM7SF1
Reactivity
Human
Synonyms
Integral Membrane Protein GPR137B (GPR137B); Human Integral Membrane Protein GPR137B (GPR137B) ELISA Kit; GPR137B; TM7SF1; transmembrane 7 superfamily member 1 (upregulated in kidney); GPR137B elisa kit
Reactivity
Human
Specificity
Human Integral membrane protein GPR137B (GPR137B) ELISA Kit has high sensitivity and excellent specificity for detection of Human GPR137B. No significant cross-reactivity or interference between Human GPR137B and analogues was observed.
Sequence Length
399
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Integral membrane protein GPR137B (GPR137B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Integral membrane protein GPR137B (GPR137B) Standard Curve.)
Related Product Information for GPR137B elisa kit
Description: This Human Integral membrane protein GPR137B (GPR137B) ELISA Kit employs a two-site sandwich ELISA to quantitate GPR137B in samples. An antibody specific for GPR137B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPR137B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR137B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR137B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TM7SF1, a gene that is transcriptionally upregulated during kidney development. RT-PCR detected 3 alternatively spliced TM7SF1 transcripts, which the authors named TM7SF1-long (TM7SF1L), TM7SF1-intermediate (TM7SF1I), and TM7SF1-short (TM7SF1S). Northern blot analysis detected an approximately 2.4-kb TM7SF1 transcript at highest levels in human kidney and heart, with lower levels detected in brain and placenta. Additional experiments found TM7SF1 expression in spinal cord, caudate nucleus, and putamen. Studies on Wilms tumor samples showed variable TM7SF1 expression, ranging from nearly undetectable levels to an abundant level of expression comparable to that of adult kidney tissue.
Background: TM7SF1, a gene that is transcriptionally upregulated during kidney development. RT-PCR detected 3 alternatively spliced TM7SF1 transcripts, which the authors named TM7SF1-long (TM7SF1L), TM7SF1-intermediate (TM7SF1I), and TM7SF1-short (TM7SF1S). Northern blot analysis detected an approximately 2.4-kb TM7SF1 transcript at highest levels in human kidney and heart, with lower levels detected in brain and placenta. Additional experiments found TM7SF1 expression in spinal cord, caudate nucleus, and putamen. Studies on Wilms tumor samples showed variable TM7SF1 expression, ranging from nearly undetectable levels to an abundant level of expression comparable to that of adult kidney tissue.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
integral membrane protein GPR137B
NCBI Official Synonym Full Names
G protein-coupled receptor 137B
NCBI Official Symbol
GPR137B
NCBI Official Synonym Symbols
TM7SF1
NCBI Protein Information
integral membrane protein GPR137B
UniProt Protein Name
Integral membrane protein GPR137B
Protein Family
UniProt Gene Name
GPR137B
UniProt Synonym Gene Names
TM7SF1
UniProt Entry Name
G137B_HUMAN
Uniprot Description
GPR137B: Belongs to the GPR137 family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1q42-q43
Cellular Component: membrane; integral to plasma membrane; lysosomal membrane
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Product Notes
The Human GPR137B gpr137b (Catalog #AAA9716050) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716050 ELISA Kit recognizes Human GPR137B. It is sometimes possible for the material contained within the vial of "Integral Membrane Protein GPR137B (GPR137B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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