Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein NPAT (NPAT) Standard Curve.)
Human Protein NPAT (NPAT) ELISA Kit | NPAT elisa kit
Human Protein NPAT (NPAT) ELISA Kit
Gene Names
NPAT; E14; p220; E14/NPAT
Reactivity
Human
Synonyms
Protein NPAT (NPAT); Human Protein NPAT (NPAT) ELISA Kit; NPAT; E14; nuclear protein; ataxia-telangiectasia locus; NPAT elisa kit
Reactivity
Human
Specificity
Human Protein NPAT (NPAT) ELISA Kit has high sensitivity and excellent specificity for detection of Human NPAT. No significant cross-reactivity or interference between Human NPAT and analogues was observed.
Sequence Length
1427
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein NPAT (NPAT) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein NPAT (NPAT) Standard Curve.)
Related Product Information for NPAT elisa kit
Description: This Human Protein NPAT (NPAT) ELISA Kit employs a two-site sandwich ELISA to quantitate NPAT in samples. An antibody specific for NPAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NPAT lies only 0.5 kb from the 5-prime end of the ATM gene and is transcribed in the opposite direction as ATM.NPAT encodes a 1,427-amino acid protein containing nuclear localization signals and target sites for phosphorylation by cyclin-dependent protein kinases associated with E2F. NPAT has a calculated molecular mass of 154,300 Da. It is relatively serine and threonine rich. The mRNA of NPAT was detected in all human tissues examined and its genomic sequence was strongly conserved through eukaryotes, suggesting that the NPAT gene may be essential for cell maintenance, i.e., a housekeeping gene. The promoter region may be shared by ATM and NPAT and that each gene may influence the expression of the other.
Background: NPAT lies only 0.5 kb from the 5-prime end of the ATM gene and is transcribed in the opposite direction as ATM.NPAT encodes a 1,427-amino acid protein containing nuclear localization signals and target sites for phosphorylation by cyclin-dependent protein kinases associated with E2F. NPAT has a calculated molecular mass of 154,300 Da. It is relatively serine and threonine rich. The mRNA of NPAT was detected in all human tissues examined and its genomic sequence was strongly conserved through eukaryotes, suggesting that the NPAT gene may be essential for cell maintenance, i.e., a housekeeping gene. The promoter region may be shared by ATM and NPAT and that each gene may influence the expression of the other.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein NPAT isoform 2
NCBI Official Synonym Full Names
nuclear protein, coactivator of histone transcription
NCBI Official Symbol
NPAT
NCBI Official Synonym Symbols
E14; p220; E14/NPAT
NCBI Protein Information
protein NPAT
UniProt Protein Name
Protein NPAT
Protein Family
UniProt Gene Name
NPAT
UniProt Synonym Gene Names
CAND3; E14; Nuclear protein of the ATM locus
Uniprot Description
Required for progression through the G1 and S phases of the cell cycle and for S phase entry. Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. Also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters.
Research Articles on NPAT
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Product Notes
The Human NPAT npat (Catalog #AAA9716024) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716024 ELISA Kit recognizes Human NPAT. It is sometimes possible for the material contained within the vial of "Protein NPAT (NPAT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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