Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) Standard Curve.)
Human T-Cell Immunoglobulin And mucin Domain-Containing Protein 4 (TIMD4) ELISA Kit | TIMD4 elisa kit
Human T-Cell Immunoglobulin And mucin Domain-Containing Protein 4 (TIMD4) ELISA Kit
Gene Names
TIMD4; TIM4; SMUCKLER
Reactivity
Human
Synonyms
T-Cell Immunoglobulin And mucin Domain-Containing Protein 4 (TIMD4); Human T-Cell Immunoglobulin And mucin Domain-Containing Protein 4 (TIMD4) ELISA Kit; TIMD4; FLJ27515; SMUCKLER; TIM4; TIMD4 elisa kit
Reactivity
Human
Specificity
Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) ELISA Kit has high sensitivity and excellent specificity for detection of Human TIMD4. No significant cross-reactivity or interference between Human TIMD4 and analogues was observed.
Sequence Length
378
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) Standard Curve.)
Related Product Information for TIMD4 elisa kit
Description: This Human T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMD4 in samples. An antibody specific for TIMD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMD4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TIM4 has an N-terminal IgV domain, followed by a long mucin stalk and a C-terminal cytoplasmic tail. The IgV domain contains an N-linked glycosylation site and a potential integrin-binding RGD motif, and the mucin stalk contains 38 potential O-linked glycosylation sites and a second N-linked glycosylation site near the membrane. The cytoplasmic domain of TIM4 lacks a conserved tyrosine kinase phosphorylation site found in other TIM family members. The antigen recognized by the antibody was identified by expression cloning as a type I transmembrane protein called Tim4. Tim4 was expressed in Mac1+ cells in various mouse tissues, including spleen, lymph nodes, and fetal liver. Tim4 bound apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain.
Background: TIM4 has an N-terminal IgV domain, followed by a long mucin stalk and a C-terminal cytoplasmic tail. The IgV domain contains an N-linked glycosylation site and a potential integrin-binding RGD motif, and the mucin stalk contains 38 potential O-linked glycosylation sites and a second N-linked glycosylation site near the membrane. The cytoplasmic domain of TIM4 lacks a conserved tyrosine kinase phosphorylation site found in other TIM family members. The antigen recognized by the antibody was identified by expression cloning as a type I transmembrane protein called Tim4. Tim4 was expressed in Mac1+ cells in various mouse tissues, including spleen, lymph nodes, and fetal liver. Tim4 bound apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
T-cell immunoglobulin and mucin domain-containing protein 4 isoform 1
NCBI Official Synonym Full Names
T cell immunoglobulin and mucin domain containing 4
NCBI Official Symbol
TIMD4
NCBI Official Synonym Symbols
TIM4; SMUCKLER
NCBI Protein Information
T-cell immunoglobulin and mucin domain-containing protein 4
UniProt Protein Name
T-cell immunoglobulin and mucin domain-containing protein 4
Protein Family
UniProt Gene Name
TIMD4
UniProt Synonym Gene Names
TIM4; TIMD-4; TIM-4
UniProt Entry Name
TIMD4_HUMAN
Uniprot Description
TIM-4: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1. Belongs to the immunoglobulin superfamily. TIM family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q33.3
Cellular Component: integral to membrane
Research Articles on TIMD4
Similar Products
Product Notes
The Human TIMD4 timd4 (Catalog #AAA9715987) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715987 ELISA Kit recognizes Human TIMD4. It is sometimes possible for the material contained within the vial of "T-Cell Immunoglobulin And mucin Domain-Containing Protein 4 (TIMD4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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