Human Nexilin (NEXN) ELISA Kit | NEXN elisa kit

Human Nexilin (NEXN) ELISA Kit

Cat. Num. AAA9715882

• Gene Names: NEXN; CMH20; NELIN
• Reactivity: Human
• Synonyms: Nexilin (NEXN); Human Nexilin (NEXN) ELISA Kit; CMD1CC; MGC104234; MGC138865; MGC138866; NELIN; nexilin; NEXN elisa kit

• Reactivity: Human
• Specificity: Human Nexilin (NEXN) ELISA Kit has high sensitivity and excellent specificity for detection of Human NEXN. No significant cross-reactivity or interference between Human NEXN and analogues was observed.
• Sequence Length: 611

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Nexilin (NEXN) Standard Curve.)

Related Product Information for NEXN elisa kit

Description: This Human Nexilin (NEXN) ELISA Kit employs a two-site sandwich ELISA to quantitate NEXN in samples. An antibody specific for NEXN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NEXN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NEXN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NEXN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The name nexilin came from the Latin word nexilis, meaning 'bound together.' Full-length b-nexilin contains an N-terminal F-actin-binding domain (ABD), followed by a spacer, a coiled-coil region, a second ABD, and a long C-terminal tail. In comparison, s-nexilin lacks the N-terminal ABD and has an insertion in the C-terminal tail. Immunofluorescence microscopy revealed that both b-nexilin and s-nexilin colocalized with F-actin at focal contacts at the ends of stress fibers. The nexilins did not localize to cell-cell junctions. Western blot analysis detected nexilin proteins with apparent molecular masses above 90 kD in rat brain, testis, and spleen and in cultured rodent fibroblasts. Neither protein was detected in liver, kidney, or epithelial cell lines.

NCBI and Uniprot Product Information

• NCBI GI #: 288856248
• NCBI GeneID: 91624
• NCBI Accession #: NP_001165780.1
• NCBI GenBank Nucleotide #: NP_001165780.1
• UniProt Accession #: Q0ZGT2
• NCBI Official Full Name: nexilin isoform 2
• NCBI Official Synonym Full Names: nexilin F-actin binding protein
• NCBI Official Symbol: NEXN
• NCBI Official Synonym Symbols: CMH20; NELIN
• NCBI Protein Information: nexilin
• UniProt Protein Name: Nexilin
• Protein Family: Nexilin
• UniProt Gene Name: NEXN

NCBI Description

This gene encodes a filamentous actin-binding protein that may function in cell adhesion and migration. Mutations in this gene have been associated with dilated cardiomyopathy, also known as CMD1CC. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010]

Uniprot Description

Involved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity.