Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Deleted in azoospermia protein 2 (DAZ2) Standard Curve.)
Human Deleted In Azoospermia Protein 2 (DAZ2) ELISA Kit | DAZ2 elisa kit
Human Deleted In Azoospermia Protein 2 (DAZ2) ELISA Kit
Gene Names
DAZ2; pDP1678
Reactivity
Human
Synonyms
Deleted In Azoospermia Protein 2 (DAZ2); Human Deleted In Azoospermia Protein 2 (DAZ2) ELISA Kit; DAZ2; MGC126442; pDP1678; DAZ2 elisa kit
Reactivity
Human
Specificity
Human Deleted in azoospermia protein 2 (DAZ2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DAZ2. No significant cross-reactivity or interference between Human DAZ2 and analogues was observed.
Sequence Length
534
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Deleted in azoospermia protein 2 (DAZ2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Deleted in azoospermia protein 2 (DAZ2) Standard Curve.)
Related Product Information for DAZ2 elisa kit
Description: This Human Deleted in azoospermia protein 2 (DAZ2) ELISA Kit employs a two-site sandwich ELISA to quantitate DAZ2 in samples. An antibody specific for DAZ2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAZ2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAZ2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAZ2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DAZ2 is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of DAZ2 also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. DAZ2 contains one copy of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms.
Background: DAZ2 is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of DAZ2 also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. DAZ2 contains one copy of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
deleted in azoospermia protein 2 isoform 2
NCBI Official Synonym Full Names
deleted in azoospermia 2
NCBI Official Symbol
DAZ2
NCBI Official Synonym Symbols
pDP1678
NCBI Protein Information
deleted in azoospermia protein 2
UniProt Protein Name
Deleted in azoospermia protein 2
Protein Family
UniProt Gene Name
DAZ2
UniProt Entry Name
DAZ2_HUMAN
NCBI Description
This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Research Articles on DAZ2
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Product Notes
The Human DAZ2 daz2 (Catalog #AAA9715804) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715804 ELISA Kit recognizes Human DAZ2. It is sometimes possible for the material contained within the vial of "Deleted In Azoospermia Protein 2 (DAZ2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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