Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-defensin 134 (DEFB134) Standard Curve.)
Human Beta-Defensin 134 (DEFB134) ELISA Kit | DEFB134 elisa kit
Human Beta-Defensin 134 (DEFB134) ELISA Kit
Reactivity
Human
Synonyms
Beta-Defensin 134 (DEFB134); Human Beta-Defensin 134 (DEFB134) ELISA Kit; DEFB134; MGC163333; MGC163335; beta-defensin 134; beta-defensin 135; DEFB134 elisa kit
Reactivity
Human
Specificity
Human Beta-defensin 134 (DEFB134) ELISA Kit has high sensitivity and excellent specificity for detection of Human DEFB134. No significant cross-reactivity or interference between Human DEFB134 and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-defensin 134 (DEFB134) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-defensin 134 (DEFB134) Standard Curve.)
Related Product Information for DEFB134 elisa kit
Description: This Human Beta-defensin 134 (DEFB134) ELISA Kit employs a two-site sandwich ELISA to quantitate DEFB134 in samples. An antibody specific for DEFB134 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFB134 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFB134 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFB134 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by DEFB134 is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with DEFB134 mapping to a cluster at 8p23.
Background: Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by DEFB134 is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with DEFB134 mapping to a cluster at 8p23.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
UniProt Protein Name
Beta-defensin 134
Protein Family
UniProt Gene Name
DEFB134
UniProt Entry Name
DB134_HUMAN
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Product Notes
The Human DEFB134 defb134 (Catalog #AAA9715768) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715768 ELISA Kit recognizes Human DEFB134. It is sometimes possible for the material contained within the vial of "Beta-Defensin 134 (DEFB134), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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