Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Laminin subunit alpha-2 (LAMA2) Standard Curve.)
Human Laminin Subunit alpha-2 (LAMA2) ELISA Kit | LAMA2 elisa kit
Human Laminin Subunit alpha-2 (LAMA2) ELISA Kit
Gene Names
LAMA2; LAMM; MDC1A
Reactivity
Human
Synonyms
Laminin Subunit alpha-2 (LAMA2); Human Laminin Subunit alpha-2 (LAMA2) ELISA Kit; LAMA2; RP1-69D17.2; LAMM; laminin M; laminin alpha 2 subunit; merosin heavy chain; LAMA2 elisa kit
Reactivity
Human
Specificity
Human Laminin subunit alpha-2 (LAMA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human LAMA2. No significant cross-reactivity or interference between Human LAMA2 and analogues was observed.
Sequence Length
3122
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Laminin subunit alpha-2 (LAMA2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Laminin subunit alpha-2 (LAMA2) Standard Curve.)
Related Product Information for LAMA2 elisa kit
Description: This Human Laminin subunit alpha-2 (LAMA2) ELISA Kit employs a two-site sandwich ELISA to quantitate LAMA2 in samples. An antibody specific for LAMA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAMA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. Laminin subunit alpha-2 is the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.
Background: Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. Laminin subunit alpha-2 is the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
laminin subunit alpha-2 isoform a
NCBI Official Synonym Full Names
laminin subunit alpha 2
NCBI Official Symbol
LAMA2
NCBI Official Synonym Symbols
LAMM; MDC1A
NCBI Protein Information
laminin subunit alpha-2
UniProt Protein Name
Laminin subunit alpha-2
Protein Family
UniProt Gene Name
LAMA2
UniProt Synonym Gene Names
LAMM
UniProt Entry Name
LAMA2_HUMAN
NCBI Description
Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Jul 2008]
Research Articles on LAMA2
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Product Notes
The Human LAMA2 lama2 (Catalog #AAA9715527) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715527 ELISA Kit recognizes Human LAMA2. It is sometimes possible for the material contained within the vial of "Laminin Subunit alpha-2 (LAMA2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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