Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucine-rich repeat LGI family member 3 (LGI3) Standard Curve.)
Human Leucine-Rich Repeat LGI Family Member 3 (LGI3) ELISA Kit | LGI3 elisa kit
Human Leucine-Rich Repeat LGI Family Member 3 (LGI3) ELISA Kit
Gene Names
LGI3; LGIL4
Reactivity
Human
Synonyms
Leucine-Rich Repeat LGI Family Member 3 (LGI3); Human Leucine-Rich Repeat LGI Family Member 3 (LGI3) ELISA Kit; LGI3; LGIL4; LGI3 elisa kit
Reactivity
Human
Specificity
Human Leucine-rich repeat LGI family member 3 (LGI3) ELISA Kit has high sensitivity and excellent specificity for detection of Human LGI3. No significant cross-reactivity or interference between Human LGI3 and analogues was observed.
Sequence Length
548
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucine-rich repeat LGI family member 3 (LGI3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucine-rich repeat LGI family member 3 (LGI3) Standard Curve.)
Related Product Information for LGI3 elisa kit
Description: This Human Leucine-rich repeat LGI family member 3 (LGI3) ELISA Kit employs a two-site sandwich ELISA to quantitate LGI3 in samples. An antibody specific for LGI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LGI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 549-amino acid protein contains a N-terminal signal peptide, a putative transmembrane region, and 1 partial and 4 complete leucine-rich repeats (LRRs). The LRRs are flanked on both sides by cysteine-rich regions, and the N-terminal cysteine-rich region contains a motif conserved in LGIs. LGI3 shares significant similarity with LGI1, LGI2, and LGI4. Semiquantitative PCR detected LGI3 expression in all tissues examined, with highest levels in brain and lung. By in situ hybridization of adult mouse brain, Senechal et al. (2005) showed that Lgi3 was expressed at low levels in most brain regions with high levels in the facial nerve nucleus. Lgi3 was secreted in transfected 293T cells.
Background: The deduced 549-amino acid protein contains a N-terminal signal peptide, a putative transmembrane region, and 1 partial and 4 complete leucine-rich repeats (LRRs). The LRRs are flanked on both sides by cysteine-rich regions, and the N-terminal cysteine-rich region contains a motif conserved in LGIs. LGI3 shares significant similarity with LGI1, LGI2, and LGI4. Semiquantitative PCR detected LGI3 expression in all tissues examined, with highest levels in brain and lung. By in situ hybridization of adult mouse brain, Senechal et al. (2005) showed that Lgi3 was expressed at low levels in most brain regions with high levels in the facial nerve nucleus. Lgi3 was secreted in transfected 293T cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
LGI3
NCBI Official Synonym Full Names
leucine rich repeat LGI family member 3
NCBI Official Symbol
LGI3
NCBI Official Synonym Symbols
LGIL4
NCBI Protein Information
leucine-rich repeat LGI family member 3
UniProt Protein Name
Leucine-rich repeat LGI family member 3
Protein Family
UniProt Gene Name
LGI3
UniProt Synonym Gene Names
LGIL4
UniProt Entry Name
LGI3_HUMAN
Uniprot Description
LGI3: May participate in the regulation of neuronal exocytosis.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 8p21.3
Cellular Component: synaptic vesicle; neuron projection; extracellular region; cell junction
Molecular Function: catalytic activity
Biological Process: regulation of exocytosis; exocytosis; metabolic process
Research Articles on LGI3
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Product Notes
The Human LGI3 lgi3 (Catalog #AAA9715482) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715482 ELISA Kit recognizes Human LGI3. It is sometimes possible for the material contained within the vial of "Leucine-Rich Repeat LGI Family Member 3 (LGI3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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