Human Myoglobin (MB) ELISA Kit | MB elisa kit

Human Myoglobin (MB) ELISA Kit

Cat. Num. AAA9715369

• Reactivity: Human
• Synonyms: Myoglobin (MB); Human Myoglobin (MB) ELISA Kit; MB; MGC13548; PVALB; OTTHUMP00000197922; MB elisa kit

• Reactivity: Human
• Specificity: Human Myoglobin (MB) ELISA Kit has high sensitivity and excellent specificity for detection of Human MB. No significant cross-reactivity or interference between Human MB and analogues was observed.
• Sequence Length: 154

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Myoglobin (MB) Standard Curve.)

Related Product Information for MB elisa kit

Description: This Human Myoglobin (MB) ELISA Kit employs a two-site sandwich ELISA to quantitate MB in samples. An antibody specific for MB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Myoglobin is a single-chain globular protein of 153 amino acids, containing a heme prosthetic group in the center around which the remaining apoprotein folds. It has eight alpha helices and a hydrophobic core. It has a molecular weight of 16,700 daltons, and is the primary oxygen-carrying pigment of muscle tissues. Unlike the blood-borne hemoglobin, to which it is structurally related, this protein does not exhibit cooperative binding of oxygen, since positive cooperativity is a property of multimeric/oligomeric proteins only. Instead, the binding of oxygen by myoglobin is unaffected by the oxygen pressure in the surrounding tissue. Myoglobin is often cited as having an "instant binding tenacity" to oxygen given its hyperbolic oxygen dissociation curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885477
• NCBI Accession #: NP_005359.1
• NCBI GenBank Nucleotide #: NP_005359.1
• UniProt Accession #: P02144
• NCBI Official Full Name: myoglobin
• UniProt Protein Name: Myoglobin
• Protein Family: Myoglobin
• UniProt Gene Name: MB
• UniProt Entry Name: MYG_HUMAN

Uniprot Description

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 22q13.1

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hormone stimulus; response to hypoxia; brown fat cell differentiation; enucleate erythrocyte differentiation