Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human M-phase phosphoprotein 8 (MPHOSPH8) Standard Curve.)
Human M-Phase Phosphoprotein 8 (MPHOSPH8) ELISA Kit | MPHOSPH8 elisa kit
Human M-Phase Phosphoprotein 8 (MPHOSPH8) ELISA Kit
Gene Names
MPHOSPH8; TWA3; mpp8; HSMPP8
Reactivity
Human
Synonyms
M-Phase Phosphoprotein 8 (MPHOSPH8); Human M-Phase Phosphoprotein 8 (MPHOSPH8) ELISA Kit; MPHOSPH8; RP11-523H24.1; FLJ35237; HSMPP8; TWA3; mpp8; M-phase phosphoprotein; mpp; MPHOSPH8 elisa kit
Reactivity
Human
Specificity
Human M-phase phosphoprotein 8 (MPHOSPH8) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPHOSPH8. No significant cross-reactivity or interference between Human MPHOSPH8 and analogues was observed.
Sequence Length
259
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human M-phase phosphoprotein 8 (MPHOSPH8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human M-phase phosphoprotein 8 (MPHOSPH8) Standard Curve.)
Related Product Information for MPHOSPH8 elisa kit
Description: This Human M-phase phosphoprotein 8 (MPHOSPH8) ELISA Kit employs a two-site sandwich ELISA to quantitate MPHOSPH8 in samples. An antibody specific for MPHOSPH8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPHOSPH8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPHOSPH8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPHOSPH8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MPHOSPH8 (M-Phase Phosphoprotein 8) is a Protein Coding gene. GO annotations related to this gene include methylated histone binding. Heterochromatin component that specifically recognizes and binds methylated Lys-9 of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene. Mediates down-regulation of CDH1 expression.
Background: MPHOSPH8 (M-Phase Phosphoprotein 8) is a Protein Coding gene. GO annotations related to this gene include methylated histone binding. Heterochromatin component that specifically recognizes and binds methylated Lys-9 of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene. Mediates down-regulation of CDH1 expression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
MPHOSPH8 protein
NCBI Official Synonym Full Names
M-phase phosphoprotein 8
NCBI Official Symbol
MPHOSPH8
NCBI Official Synonym Symbols
TWA3; mpp8; HSMPP8
NCBI Protein Information
M-phase phosphoprotein 8
UniProt Protein Name
M-phase phosphoprotein 8
Protein Family
UniProt Gene Name
MPHOSPH8
UniProt Synonym Gene Names
Twa31 PublicationManual assertion based on opinion iniRef.7
Uniprot Description
Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592).
Research Articles on MPHOSPH8
Similar Products
Product Notes
The Human MPHOSPH8 mphosph8 (Catalog #AAA9715228) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715228 ELISA Kit recognizes Human MPHOSPH8. It is sometimes possible for the material contained within the vial of "M-Phase Phosphoprotein 8 (MPHOSPH8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
