Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human N-acetyltransferase 15 (NAT15) Standard Curve.)
Human N-Acetyltransferase 15 (NAT15) ELISA Kit | NAT15 elisa kit
Human N-Acetyltransferase 15 (NAT15) ELISA Kit
Gene Names
NAA60; HAT4; NatF; NAT15; hNaa60
Reactivity
Human
Synonyms
N-Acetyltransferase 15 (NAT15); Human N-Acetyltransferase 15 (NAT15) ELISA Kit; NAT15; FLJ11693; FLJ14154; N-acetyltransferase 15; NAT15 elisa kit
Reactivity
Human
Specificity
Human N-acetyltransferase 15 (NAT15) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAT15. No significant cross-reactivity or interference between Human NAT15 and analogues was observed.
Sequence Length
242
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human N-acetyltransferase 15 (NAT15) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human N-acetyltransferase 15 (NAT15) Standard Curve.)
Related Product Information for NAT15 elisa kit
Description: This Human N-acetyltransferase 15 (NAT15) ELISA Kit employs a two-site sandwich ELISA to quantitate NAT15 in samples. An antibody specific for NAT15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAT15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAT15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NAT15 encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele.
Background: NAT15 encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
N-alpha-acetyltransferase 60 isoform a
NCBI Official Synonym Full Names
N(alpha)-acetyltransferase 60, NatF catalytic subunit
NCBI Official Symbol
NAA60
NCBI Official Synonym Symbols
HAT4; NatF; NAT15; hNaa60
NCBI Protein Information
N-alpha-acetyltransferase 60
UniProt Protein Name
N-alpha-acetyltransferase 60
UniProt Gene Name
NAA60
UniProt Synonym Gene Names
HAT4; NAT15; UNQ2771/PRO7155; HAT4
UniProt Entry Name
NAA60_HUMAN
NCBI Description
This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele. [provided by RefSeq, Nov 2015]
Research Articles on NAT15
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Product Notes
The Human NAT15 naa60 (Catalog #AAA9715031) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715031 ELISA Kit recognizes Human NAT15. It is sometimes possible for the material contained within the vial of "N-Acetyltransferase 15 (NAT15), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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