Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Probable histone-lysine N-methyltransferase PRDM7 (PRDM7) Standard Curve.)
Human Probable Histone-Lysine N-Methyltransferase PRDM7 (PRDM7) ELISA Kit | PRDM7 elisa kit
Human Probable Histone-Lysine N-Methyltransferase PRDM7 (PRDM7) ELISA Kit
Gene Names
PRDM7; PFM4; ZNF910
Reactivity
Human
Synonyms
Probable Histone-Lysine N-Methyltransferase PRDM7 (PRDM7); Human Probable Histone-Lysine N-Methyltransferase PRDM7 (PRDM7) ELISA Kit; PRDM7; MGC129525; PFM4; PR-domain family protein 4; PRDM7 elisa kit
Reactivity
Human
Specificity
Human Probable histone-lysine N-methyltransferase PRDM7 (PRDM7) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRDM7. No significant cross-reactivity or interference between Human PRDM7 and analogues was observed.
Sequence Length
492
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Probable histone-lysine N-methyltransferase PRDM7 (PRDM7) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Probable histone-lysine N-methyltransferase PRDM7 (PRDM7) Standard Curve.)
Related Product Information for PRDM7 elisa kit
Description: This Human Probable histone-lysine N-methyltransferase PRDM7 (PRDM7) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDM7 in samples. An antibody specific for PRDM7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDM7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Progressive diversification of paralogs after gene expansion is essential to increase their functional specialization. However, mode and tempo of this divergence remain mostly unclear. PRDM7 underwent major structural rearrangements that decreased the number of encoded Zn-Fingers and modified gene splicing. Through internal duplication and activation of a non-canonical splice site (GC-AG), PRDM7 can acquire a novel intron. PRDM7 is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Background: Progressive diversification of paralogs after gene expansion is essential to increase their functional specialization. However, mode and tempo of this divergence remain mostly unclear. PRDM7 underwent major structural rearrangements that decreased the number of encoded Zn-Fingers and modified gene splicing. Through internal duplication and activation of a non-canonical splice site (GC-AG), PRDM7 can acquire a novel intron. PRDM7 is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
probable histone-lysine N-methyltransferase PRDM7
NCBI Official Synonym Full Names
PR/SET domain 7
NCBI Official Symbol
PRDM7
NCBI Official Synonym Symbols
PFM4; ZNF910
NCBI Protein Information
probable histone-lysine N-methyltransferase PRDM7
UniProt Protein Name
Probable histone-lysine N-methyltransferase PRDM7
Protein Family
UniProt Gene Name
PRDM7
UniProt Synonym Gene Names
PFM4
NCBI Description
This gene encodes a member of a family of proteins that may have roles in transcription and other nuclear processes. The encoded protein contains a KRAB (Kruppel-associated box) domain -A box and a SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain and may function as a histone methyltransferase. [provided by RefSeq, Aug 2013]
Uniprot Description
PRDM7: a probable histone methyltransferase. The mouse orthologous protein seems not to exist. Human PRDM7 and PRDM9 genes, a pair of close paralogs corresponding to a single mouse gene Prdm9, may have been generated by a recent gene duplication event after the divergence of the ancestors of human and mouse. Three isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine
Chromosomal Location of Human Ortholog: 16q24.3
Cellular Component: chromosome; nucleus
Molecular Function: histone-lysine N-methyltransferase activity; nucleic acid binding; protein binding
Biological Process: regulation of transcription, DNA-templated
Research Articles on PRDM7
Similar Products
Product Notes
The Human PRDM7 prdm7 (Catalog #AAA9714850) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714850 ELISA Kit recognizes Human PRDM7. It is sometimes possible for the material contained within the vial of "Probable Histone-Lysine N-Methyltransferase PRDM7 (PRDM7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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