Human Histone-Lysine N-Methyltransferase PRDM9 (PRDM9) ELISA Kit | PRDM9 elisa kit

Human Histone-Lysine N-Methyltransferase PRDM9 (PRDM9) ELISA Kit

Cat. Num. AAA9714848

• Gene Names: PRDM9; PFM6; KMT8B; MSBP3; ZNF899; MEISETZ
• Reactivity: Human
• Synonyms: Histone-Lysine N-Methyltransferase PRDM9 (PRDM9); Human Histone-Lysine N-Methyltransferase PRDM9 (PRDM9) ELISA Kit; PRDM9; PFM6; PR-domain containing protein 9; PRDM9 elisa kit

• Reactivity: Human
• Specificity: Human Histone-lysine N-methyltransferase PRDM9 (PRDM9) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRDM9. No significant cross-reactivity or interference between Human PRDM9 and analogues was observed.
• Sequence Length: 894

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Histone-lysine N-methyltransferase PRDM9 (PRDM9) Standard Curve.)

Related Product Information for PRDM9 elisa kit

Description: This Human Histone-lysine N-methyltransferase PRDM9 (PRDM9) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDM9 in samples. An antibody specific for PRDM9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDM9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The PR domain is a protein-protein interaction module of about 100 amino acids. PR domain-containing proteins, such as PRDM9, are often involved in transcriptional regulation. The deduced 862-amino acid Meisetz protein contains a PR/SET domain in its N-terminal portion and a zinc finger domain containing multiple C2H2-type zinc fingers in its C-terminal portion. Hayashi et al. (2005) also cloned 2 Meisetz splice variants that encode shorter isoforms lacking the zinc finger domain. The PR/SET domain of Meisetz is highly conserved in human PRDM9. In situ hybridization, RT-PCR, and Northern blot analysis showed that mouse Meisetz transcripts were only expressed in germ cells entering meiotic prophase in female fetal gonads and in postnatal testis.

NCBI and Uniprot Product Information

• NCBI GI #: 147905620
• NCBI GeneID: 56979
• NCBI Accession #: NP_064612.2
• NCBI GenBank Nucleotide #: NP_064612.2
• UniProt Accession #: Q9NQV7
• NCBI Official Full Name: histone-lysine N-methyltransferase PRDM9 isoform PRDM9 B
• NCBI Official Synonym Full Names: PR/SET domain 9
• NCBI Official Symbol: PRDM9
• NCBI Official Synonym Symbols: PFM6; KMT8B; MSBP3; ZNF899; MEISETZ
• NCBI Protein Information: histone-lysine N-methyltransferase PRDM9
• UniProt Protein Name: Histone-lysine N-methyltransferase PRDM9
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: PRDM9
• UniProt Synonym Gene Names: PFM6
• UniProt Entry Name: PRDM9_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described. [provided by RefSeq, Jul 2015]