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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Ras-related protein Rap-1b (RAP1B) Standard Curve.)

Human Ras-Related Protein Rap-1b (RAP1B) ELISA Kit | RAP1B elisa kit

Human Ras-Related Protein Rap-1b (RAP1B) ELISA Kit

Gene Names
RAP1B; K-REV; RAL1B
Reactivity
Human
Synonyms
Ras-Related Protein Rap-1b (RAP1B); Human Ras-Related Protein Rap-1b (RAP1B) ELISA Kit; RAP1B; DKFZp586H0723; K-REV; RAL1B; RAS-related protein RAP1B; Ras family small GTP binding protein RAP1B; small GTP binding protein; RAP1B elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Ras-related protein Rap-1b (RAP1B) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAP1B. No significant cross-reactivity or interference between Human RAP1B and analogues was observed.
Sequence Length
184
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Ras-related protein Rap-1b (RAP1B) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Ras-related protein Rap-1b (RAP1B) Standard Curve.)
Related Product Information for RAP1B elisa kit
Description: This Human Ras-related protein Rap-1b (RAP1B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAP1B in samples. An antibody specific for RAP1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAP1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAP1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAP1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The deduced 184-amino acid protein is 95% identical to RAP1A. RAP1B shares several properties with the RAS protein, suggesting that it may bind GTP/GDP and have a membrane location.RAP1B is mainly located at the membrane, but translocates to the cytosol upon activation. In activated platelets, RAP1B interacts with the reorganized actin-based cytoskeleton. RAP1B is activated by phosphorylation, increased intracellular Ca(2+), and by agonist-induced stimulation of Gi, which results in the rapid binding of GTP to RAP1B. RAP1B is posttranslationally modified by the geranylgeranylation of cys181. Further modifications cause proteolytic removal of the last 3 C-terminal amino acids, followed by partial methylation of the remaining terminal cysteinyl carboxyl group.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Ras-related protein Rap-1b
NCBI Official Synonym Full Names
RAP1B, member of RAS oncogene family
NCBI Official Symbol
RAP1B
NCBI Official Synonym Symbols
K-REV; RAL1B
NCBI Protein Information
ras-related protein Rap-1b
UniProt Protein Name
Ras-related protein Rap-1b
Protein Family
UniProt Gene Name
RAP1B
UniProt Entry Name
RAP1B_HUMAN

NCBI Description

This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9. [provided by RefSeq, Oct 2011]

Uniprot Description

RAP1B: GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Heterodimer with RAP1GAP. Interacts with EPAC2, SGSM1, SGSM2 and SGSM3. Activated by binding to the GTPase-activating protein RAP1GAP. Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner. Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein, monomeric, Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: membrane; plasma membrane; lipid particle; intracellular; intercellular junction; cytosol; lipid raft

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; protein complex binding

Biological Process: platelet activation; cell proliferation; microvillus biogenesis; negative regulation of calcium ion-dependent exocytosis; metabolic process; blood coagulation; Rap protein signal transduction

Research Articles on RAP1B

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Product Notes

The Human RAP1B rap1b (Catalog #AAA9714644) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714644 ELISA Kit recognizes Human RAP1B. It is sometimes possible for the material contained within the vial of "Ras-Related Protein Rap-1b (RAP1B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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