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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Adipose differentiation-related protein (ADRP) Standard Curve.)

Human Adipose Differentiation-Related Protein (ADRP) ELISA Kit | ADRP elisa kit

Human Adipose Differentiation-Related Protein (ADRP) ELISA Kit

Gene Names
PLIN2; ADFP; ADRP
Reactivity
Human
Synonyms
Adipose Differentiation-Related Protein (ADRP); Human Adipose Differentiation-Related Protein (ADRP) ELISA Kit; ADFP; RP11-151J10.1; ADRP; MGC10598; adipophilin; adipose differentiation-related protein; ADRP elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Adipose differentiation-related protein (ADRP) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADFP. No significant cross-reactivity or interference between Human ADFP and analogues was observed.
Sequence Length
437
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Adipose differentiation-related protein (ADRP) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Adipose differentiation-related protein (ADRP) Standard Curve.)
Related Product Information for ADRP elisa kit
Description: This Human Adipose differentiation-related protein (ADRP) ELISA Kit employs a two-site sandwich ELISA to quantitate ADFP in samples. An antibody specific for ADFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADFP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADFP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Adipose differentiation-related protein, also known as ADFP or adipophilin, is a protein encoded by the ADFP gene.Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation Perilipin is part of a gene family with five currently-known members. In vertebrates, closely related genes include adipophilin (also known as adipose differentiation-related protein), TIP47, and LSDP5 (also called MLDP and OXPAT). Insects express related proteins, LSD1 and LSD2, in fat bodies.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
123
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
perilipin-2
NCBI Official Synonym Full Names
perilipin 2
NCBI Official Symbol
PLIN2
NCBI Official Synonym Symbols
ADFP; ADRP
NCBI Protein Information
perilipin-2
UniProt Protein Name
Perilipin-2
UniProt Gene Name
PLIN2
UniProt Synonym Gene Names
ADFP; ADRP
UniProt Entry Name
PLIN2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

perilipin 2: May be involved in development and maintenance of adipose tissue. Belongs to the perilipin family.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: endoplasmic reticulum; extracellular region; plasma membrane; lipid particle; nucleus; cytosol

Biological Process: long-chain fatty acid transport; response to drug; sequestering of lipid; cellular lipid metabolic process; response to organic cyclic substance

Research Articles on ADRP

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Product Notes

The Human ADRP plin2 (Catalog #AAA9714599) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714599 ELISA Kit recognizes Human ADRP. It is sometimes possible for the material contained within the vial of "Adipose Differentiation-Related Protein (ADRP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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