Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human ATP-binding cassette sub-family C member 8 (ABCC8) Standard Curve.)
Human ATP-Binding Cassette Sub-Family C Member 8 (ABCC8) ELISA Kit | ABCC8 elisa kit
Human ATP-Binding Cassette Sub-Family C Member 8 (ABCC8) ELISA Kit
Gene Names
ABCC8; HI; SUR; HHF1; MRP8; PHHI; SUR1; ABC36; HRINS; TNDM2; SUR1delta2
Reactivity
Human
Synonyms
ATP-Binding Cassette Sub-Family C Member 8 (ABCC8); Human ATP-Binding Cassette Sub-Family C Member 8 (ABCC8) ELISA Kit; ABCC8; ABC36; HHF1; HI; HRINS; MRP8; PHHI; SUR; SUR1; TNDM2; ATP-binding cassette; sub-family C; member 8; sulfonylurea receptor (hyperinsulinemia); ABCC8 elisa kit
Reactivity
Human
Specificity
Human ATP-binding cassette sub-family C member 8 (ABCC8) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABCC8. No significant cross-reactivity or interference between Human ABCC8 and analogues was observed.
Sequence Length
1581
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human ATP-binding cassette sub-family C member 8 (ABCC8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human ATP-binding cassette sub-family C member 8 (ABCC8) Standard Curve.)
Related Product Information for ABCC8 elisa kit
Description: This Human ATP-binding cassette sub-family C member 8 (ABCC8) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC8 in samples. An antibody specific for ABCC8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABCC8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ABCC8 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion.
Background: ABCC8 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ATP-binding cassette sub-family C member 8 isoform 2
NCBI Official Synonym Full Names
ATP binding cassette subfamily C member 8
NCBI Official Symbol
ABCC8
NCBI Official Synonym Symbols
HI; SUR; HHF1; MRP8; PHHI; SUR1; ABC36; HRINS; TNDM2; SUR1delta2
NCBI Protein Information
ATP-binding cassette sub-family C member 8
UniProt Protein Name
ATP-binding cassette sub-family C member 8
Protein Family
UniProt Gene Name
ABCC8
UniProt Synonym Gene Names
HRINS; SUR; SUR1
NCBI Description
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
Uniprot Description
Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K+ channels and insulin release.
Research Articles on ABCC8
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Product Notes
The Human ABCC8 abcc8 (Catalog #AAA9714528) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714528 ELISA Kit recognizes Human ABCC8. It is sometimes possible for the material contained within the vial of "ATP-Binding Cassette Sub-Family C Member 8 (ABCC8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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