Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ribonuclease, RNase A family 2 (RNASE2) Standard Curve.)
Human Ribonuclease, RNase A Family 2 (RNASE2) ELISA Kit | RNASE2 elisa kit
Human Ribonuclease, RNase A Family 2 (RNASE2) ELISA Kit
Gene Names
RNASE2; EDN; RAF3; RNS2
Reactivity
Human
Synonyms
Ribonuclease; RNase A Family 2 (RNASE2); Human Ribonuclease; RNase A Family 2 (RNASE2) ELISA Kit; RNASE2; EDN; RNS2; RNASE2 elisa kit
Reactivity
Human
Specificity
Human Ribonuclease, RNase A family 2 (RNASE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human RNASE2. No significant cross-reactivity or interference between Human RNASE2 and analogues was observed.
Sequence Length
161
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ribonuclease, RNase A family 2 (RNASE2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ribonuclease, RNase A family 2 (RNASE2) Standard Curve.)
Related Product Information for RNASE2 elisa kit
Description: This Human Ribonuclease, RNase A family 2 (RNASE2) ELISA Kit employs a two-site sandwich ELISA to quantitate RNASE2 in samples. An antibody specific for RNASE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RNASE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Eosinophil-derived neurotoxin is a distinct cationic protein of the eosinophil's large specific granule known primarily for its ability to induce ataxia, paralysis, and central nervous system cellular degeneration in experimental animals (Gordon phenomenon). Rosenberg et al. (1989) isolated a 725-bp cDNA clone for EDN. The open reading frame encodes a 134-amino acid polypeptide with a molecular mass of 15.5 kD and a 27-residue N-terminal hydrophobic leader sequence. The sequence of the mature polypeptide was identical to that reported for human urinary ribonuclease and to the N-terminal sequence of human liver ribonuclease. Similarities to the ribonucleases of pancreas and angiogenin indicate that EDN belongs to the ribonuclease multigene family. This gene is also symbolized RNS2 for ribonuclease 2.
Background: Eosinophil-derived neurotoxin is a distinct cationic protein of the eosinophil's large specific granule known primarily for its ability to induce ataxia, paralysis, and central nervous system cellular degeneration in experimental animals (Gordon phenomenon). Rosenberg et al. (1989) isolated a 725-bp cDNA clone for EDN. The open reading frame encodes a 134-amino acid polypeptide with a molecular mass of 15.5 kD and a 27-residue N-terminal hydrophobic leader sequence. The sequence of the mature polypeptide was identical to that reported for human urinary ribonuclease and to the N-terminal sequence of human liver ribonuclease. Similarities to the ribonucleases of pancreas and angiogenin indicate that EDN belongs to the ribonuclease multigene family. This gene is also symbolized RNS2 for ribonuclease 2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
eosinophil-derived neurotoxin
NCBI Official Synonym Full Names
ribonuclease A family member 2
NCBI Official Symbol
RNASE2
NCBI Official Synonym Symbols
EDN; RAF3; RNS2
NCBI Protein Information
non-secretory ribonuclease
UniProt Protein Name
Non-secretory ribonuclease
Protein Family
UniProt Gene Name
RNASE2
UniProt Synonym Gene Names
EDN; RNS2; RNase 2
UniProt Entry Name
RNAS2_HUMAN
NCBI Description
The protein encoded by this gene is a non-secretory ribonuclease that belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein antimicrobial activity against viruses. [provided by RefSeq, Oct 2014]
Uniprot Description
RNASE2: This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities. Belongs to the pancreatic ribonuclease family.
Protein type: Motility/polarity/chemotaxis; Ribonuclease; EC 3.1.27.5; RNA processing
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: lysosome; extracellular region
Molecular Function: nucleic acid binding; pancreatic ribonuclease activity; ribonuclease activity
Biological Process: RNA catabolic process; chemotaxis
Research Articles on RNASE2
Similar Products
Product Notes
The Human RNASE2 rnase2 (Catalog #AAA9714453) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714453 ELISA Kit recognizes Human RNASE2. It is sometimes possible for the material contained within the vial of "Ribonuclease, RNase A Family 2 (RNASE2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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