Typical Testing Data/Standard Curve (for reference only)
Bovine Non-secretory ribonuclease (RNASE2) ELISA Kit | RNASE2 elisa kit
Bovine Non-secretory ribonuclease (RNASE2) ELISA Kit
Gene Names
RNASE2; EDN; RAF3; RNS2
Reactivity
Bovine
Synonyms
Non-secretory ribonuclease (RNASE2); Bovine Non-secretory ribonuclease (RNASE2) ELISA Kit; RNASE2 elisa kit
Reactivity
Bovine
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RNASE2 elisa kit
Intended Uses: This RNA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanRNA. This ELISA kit for research use only!
Principle of the Assay: RNA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RNA antibody and an RNA-HRP conjugate. The assay sample and buffer are incubated together with RNA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RNA concentration since RNA from samples and RNA-HRP conjugate compete for the anti-RNA antibody binding site. Since the number of sites is limited, as more sites are occupied by RNA from the sample, fewer sites are left to bind RNA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNA concentration in each sample is interpolated from this standard curve.
Principle of the Assay: RNA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RNA antibody and an RNA-HRP conjugate. The assay sample and buffer are incubated together with RNA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RNA concentration since RNA from samples and RNA-HRP conjugate compete for the anti-RNA antibody binding site. Since the number of sites is limited, as more sites are occupied by RNA from the sample, fewer sites are left to bind RNA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNA concentration in each sample is interpolated from this standard curve.
Product Categories/Family for RNASE2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,354 Da
NCBI Official Full Name
non-secretory ribonuclease
NCBI Official Synonym Full Names
ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin)
NCBI Official Symbol
RNASE2
NCBI Official Synonym Symbols
EDN; RAF3; RNS2
NCBI Protein Information
non-secretory ribonuclease; RNase 2; RNase UpI-2; ribonuclease 2; ribonuclease US; ribonuclease A F3; eosinophil-derived neurotoxin
UniProt Protein Name
Non-secretory ribonuclease
Protein Family
UniProt Gene Name
RNASE2
UniProt Synonym Gene Names
EDN; RNS2; RNase 2
UniProt Entry Name
RNAS2_HUMAN
NCBI Description
The protein encoded by this gene is a non-secretory ribonuclease that belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein antimicrobial activity against viruses. [provided by RefSeq, Oct 2014]
Uniprot Description
RNASE2: This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities. Belongs to the pancreatic ribonuclease family.
Protein type: EC 3.1.27.5; RNA processing; Ribonuclease; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: lysosome; extracellular region
Molecular Function: nucleic acid binding; pancreatic ribonuclease activity; ribonuclease activity
Biological Process: RNA catabolic process; chemotaxis
Research Articles on RNASE2
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Product Notes
The Bovine RNASE2 rnase2 (Catalog #AAA7231442) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7231442 ELISA Kit recognizes Bovine RNASE2. It is sometimes possible for the material contained within the vial of "Non-secretory ribonuclease (RNASE2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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