Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Amiloride-sensitive sodium channel subunit alpha (SCNN1A) Standard Curve.)
Human Amiloride-Sensitive Sodium Channel Subunit alpha (SCNN1A) ELISA Kit | SCNN1A elisa kit
Human Amiloride-Sensitive Sodium Channel Subunit alpha (SCNN1A) ELISA Kit
Gene Names
SCNN1A; BESC2; ENaCa; SCNEA; SCNN1; LIDLS3; ENaCalpha
Reactivity
Human
Synonyms
Amiloride-Sensitive Sodium Channel Subunit alpha (SCNN1A); Human Amiloride-Sensitive Sodium Channel Subunit alpha (SCNN1A) ELISA Kit; SCNN1A; BESC2; ENaCa; ENaCalpha; FLJ21883; SCNEA; SCNN1; alpha ENaC-2; amiloride-sensitive epithelial sodium channel alpha subunit; amiloride-sensitive sodium channel subunit alpha 2; nasal epithelial sodium c; SCNN1A elisa kit
Reactivity
Human
Specificity
Human Amiloride-sensitive sodium channel subunit alpha (SCNN1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCNN1A. No significant cross-reactivity or interference between Human SCNN1A and analogues was observed.
Sequence Length
669
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Amiloride-sensitive sodium channel subunit alpha (SCNN1A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Amiloride-sensitive sodium channel subunit alpha (SCNN1A) Standard Curve.)
Related Product Information for SCNN1A elisa kit
Description: This Human Amiloride-sensitive sodium channel subunit alpha (SCNN1A) ELISA Kit employs a two-site sandwich ELISA to quantitate SCNN1A in samples. An antibody specific for SCNN1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCNN1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCNN1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCNN1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. Amiloride Sensitive Sodium Channel Subunit Alpha is the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart.
Background: Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. Amiloride Sensitive Sodium Channel Subunit Alpha is the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
amiloride-sensitive sodium channel subunit alpha isoform 1
NCBI Official Synonym Full Names
sodium channel epithelial 1 alpha subunit
NCBI Official Symbol
SCNN1A
NCBI Official Synonym Symbols
BESC2; ENaCa; SCNEA; SCNN1; LIDLS3; ENaCalpha
NCBI Protein Information
amiloride-sensitive sodium channel subunit alpha
UniProt Protein Name
Amiloride-sensitive sodium channel subunit alpha
Protein Family
UniProt Gene Name
SCNN1A
UniProt Synonym Gene Names
SCNN1; Alpha-ENaC; ENaCA
UniProt Entry Name
SCNNA_HUMAN
NCBI Description
Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009]
Research Articles on SCNN1A
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Product Notes
The Human SCNN1A scnn1a (Catalog #AAA9714402) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714402 ELISA Kit recognizes Human SCNN1A. It is sometimes possible for the material contained within the vial of "Amiloride-Sensitive Sodium Channel Subunit alpha (SCNN1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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