Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human NAD-dependent deacetylase sirtuin-6 (SIRT6/SIR2L6) Standard Curve.)
Human NAD-Dependent Deacetylase Sirtuin-6 (SIRT6/SIR2L6) ELISA Kit | SIRT6 elisa kit
Human NAD-Dependent Deacetylase Sirtuin-6 (SIRT6/SIR2L6) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human NAD-dependent deacetylase sirtuin-6 (SIRT6/SIR2L6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human NAD-dependent deacetylase sirtuin-6 (SIRT6/SIR2L6) Standard Curve.)
Background: SIRT6 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.Sirtuins can be divided into 4 main classes in eukaryotes based on amino acid sequence motifs, with yeast Sir2 belonging to class Ia. The deduced 355-amino acid SIRT6 protein is a class IV sirtuin, a class not present in prokaryotes.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
Uniprot Description
SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Deacetylase; EC 2.4.2.31; EC 3.5.1.-; Transferase
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nuclear telomeric heterochromatin; nucleolus; nucleus
Molecular Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); zinc ion binding; NAD-dependent histone deacetylase activity; chromatin binding; NAD+ ADP-ribosyltransferase activity
Biological Process: protein amino acid ADP-ribosylation; histone deacetylation
Research Articles on SIRT6
Similar Products
Product Notes
The Human SIRT6 sirt6 (Catalog #AAA9714314) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714314 ELISA Kit recognizes Human SIRT6. It is sometimes possible for the material contained within the vial of "NAD-Dependent Deacetylase Sirtuin-6 (SIRT6/SIR2L6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
