Human Trinucleotide Repeat-Containing Gene 6B Protein (TNRC6B) ELISA Kit | TNRC6B elisa kit

Human Trinucleotide Repeat-Containing Gene 6B Protein (TNRC6B) ELISA Kit

Cat. Num. AAA9713844

• Reactivity: Human
• Synonyms: Trinucleotide Repeat-Containing Gene 6B Protein (TNRC6B); Human Trinucleotide Repeat-Containing Gene 6B Protein (TNRC6B) ELISA Kit; TNRC6B; RP5-1042K10.7; KIAA1093; OTTHUMP00000199175; TNRC6B elisa kit

• Reactivity: Human
• Specificity: Human Trinucleotide repeat-containing gene 6B protein (TNRC6B) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNRC6B. No significant cross-reactivity or interference between Human TNRC6B and analogues was observed.
• Sequence Length: 1029

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Trinucleotide repeat-containing gene 6B protein (TNRC6B) Standard Curve.)

Related Product Information for TNRC6B elisa kit

Description: This Human Trinucleotide repeat-containing gene 6B protein (TNRC6B) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC6B in samples. An antibody specific for TNRC6B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNRC6B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC6B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC6B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The deduced 1,203-amino acid protein contains an RNA recognition motif. RT-PCR ELISA detected moderate expression in all tissues and specific brain regions examined, with highest expression in cerebellum. Schneider et al. (2006) stated that the 1,727-amino acid TNRC6B protein contains a glycine/tryptophan (GW)-rich N-terminal domain, a central glutamine-rich region, and a C-terminal RNA recognition motif. TNRC6B copurified with a cytoplasmic HeLa cell protein complex containing AGO2 (EIF2C2), DICER, and MOV10. Epitope-tagged TNRC6B coimmunoprecipitated with tagged AGO1 (EIF2C1) and tagged AGO2. TNRC6B was required to mediate microRNA-guided mRNA cleavage in HeLa cell culture.

NCBI and Uniprot Product Information

• NCBI GI #: 20306948
• NCBI GeneID: 23112
• UniProt Accession #: Q9UPQ9
• NCBI Official Full Name: TNRC6B protein
• NCBI Official Synonym Full Names: trinucleotide repeat containing adaptor 6B
• NCBI Official Symbol: TNRC6B
• NCBI Protein Information: trinucleotide repeat-containing gene 6B protein
• UniProt Protein Name: Trinucleotide repeat-containing gene 6B protein
• Protein Family: Trinucleotide repeat-containing gene 6B protein
• UniProt Gene Name: TNRC6B
• UniProt Synonym Gene Names: KIAA1093
• UniProt Entry Name: TNR6B_HUMAN

Uniprot Description

Function: Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes. Ref.7 Ref.10 Ref.11 Ref.16

Subunit structure: Interacts with AGO1, AGO2, AGO3 and AGO4. Interacts with CNOT1; the interaction mediates the association with the CCR4-NOT complex. Interacts with PAN3; the interaction mediates the association with the PAN complex. Ref.7 Ref.8 Ref.10 Ref.11 Ref.12 Ref.16 Ref.17

Subcellular location: Cytoplasm › P-body. Note: Mammalian P-bodies are also known as GW bodies (GWBs). Ref.7 Ref.10

Sequence similarities: Belongs to the GW182 family.Contains 1 RRM (RNA recognition motif) domain.

Sequence caution: The sequence BAA83045.2 differs from that shown. Reason: Erroneous initiation.