Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Zymogen granule protein 16 homolog B (ZG16B) Standard Curve.)
Human Zymogen Granule Protein 16 Homolog B (ZG16B) ELISA Kit | ZG16B elisa kit
Human Zymogen Granule Protein 16 Homolog B (ZG16B) ELISA Kit
Gene Names
ZG16B; EECP; PAUF; JCLN2; HRPE773; PRO1567
Reactivity
Human
Synonyms
Zymogen Granule Protein 16 Homolog B (ZG16B); Human Zymogen Granule Protein 16 Homolog B (ZG16B) ELISA Kit; ZG16B; HRPE773; JCLN2; PAUF; PRO1567; jacalin-like lectin domain containing 2; pancreatic adenocarcinoma upregulated factor; zymogen granule protein 16 homolog B; ZG16B elisa kit
Reactivity
Human
Specificity
Human Zymogen granule protein 16 homolog B (ZG16B) ELISA Kit has high sensitivity and excellent specificity for detection of Human ZG16B. No significant cross-reactivity or interference between Human ZG16B and analogues was observed.
Sequence Length
208
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Zymogen granule protein 16 homolog B (ZG16B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Zymogen granule protein 16 homolog B (ZG16B) Standard Curve.)
Related Product Information for ZG16B elisa kit
Description: This Human Zymogen granule protein 16 homolog B (ZG16B) ELISA Kit employs a two-site sandwich ELISA to quantitate ZG16B in samples. An antibody specific for ZG16B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZG16B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZG16B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZG16B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ZG16b/PAUF has recently been found to play a role in gene regulation and cancer metastasis. However, the detailed functions of ZG16p and ZG16b remain to be clarified. ZG16p has a Jacalin-related beta-prism fold, the first to be reported among mammalian lectins. The putative sugar-binding site of ZG16p is occupied by a glycerol molecule, mimicking the mannose bound to Jacalin-related mannose-binding-type plant lectins such as Banlec. ZG16b also has a beta-prism fold, but some amino acid residues of the putative sugar-binding site differ from those of the mannose-type binding site suggesting altered preference. A positively charged patch, which may bind sulfated groups of the glycosaminoglycans, is located around the putative sugar-binding site of ZG16p and ZG16b. The sugar-binding site and the adjacent basic patch of ZG16p and ZG16b cooperatively form a functional glycosaminoglycan-binding site.
Background: ZG16b/PAUF has recently been found to play a role in gene regulation and cancer metastasis. However, the detailed functions of ZG16p and ZG16b remain to be clarified. ZG16p has a Jacalin-related beta-prism fold, the first to be reported among mammalian lectins. The putative sugar-binding site of ZG16p is occupied by a glycerol molecule, mimicking the mannose bound to Jacalin-related mannose-binding-type plant lectins such as Banlec. ZG16b also has a beta-prism fold, but some amino acid residues of the putative sugar-binding site differ from those of the mannose-type binding site suggesting altered preference. A positively charged patch, which may bind sulfated groups of the glycosaminoglycans, is located around the putative sugar-binding site of ZG16p and ZG16b. The sugar-binding site and the adjacent basic patch of ZG16p and ZG16b cooperatively form a functional glycosaminoglycan-binding site.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
zymogen granule protein 16 homolog B
NCBI Official Synonym Full Names
zymogen granule protein 16B
NCBI Official Symbol
ZG16B
NCBI Official Synonym Symbols
EECP; PAUF; JCLN2; HRPE773; PRO1567
NCBI Protein Information
zymogen granule protein 16 homolog B
UniProt Protein Name
Zymogen granule protein 16 homolog B
Protein Family
UniProt Gene Name
ZG16B
Uniprot Description
CautionIt is uncertain whether Met-1, Met-13 or Met-31 is the initiator.
Research Articles on ZG16B
Similar Products
Product Notes
The Human ZG16B zg16b (Catalog #AAA9713671) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713671 ELISA Kit recognizes Human ZG16B. It is sometimes possible for the material contained within the vial of "Zymogen Granule Protein 16 Homolog B (ZG16B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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