Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) Standard Curve.)
Rat 5'-Nucleotidase/Cluster Of Differentiation 73 (NT5E/CD73) ELISA Kit | NT5E/CD73 elisa kit
Rat 5'-Nucleotidase/Cluster Of Differentiation 73 (NT5E/CD73) ELISA Kit
Reactivity
Rat
Synonyms
5'-Nucleotidase/Cluster Of Differentiation 73 (NT5E/CD73); Rat 5'-Nucleotidase/Cluster Of Differentiation 73 (NT5E/CD73) ELISA Kit; NT5E/CD73 elisa kit
Reactivity
Rat
Specificity
Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NT5E/CD73. No significant cross-reactivity or interference between Rat NT5E/CD73 and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) Standard Curve.)
Related Product Information for NT5E/CD73 elisa kit
Description: This Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA Kit employs a two-site sandwich ELISA to quantitate NT5E/CD73 in samples. An antibody specific for NT5E/CD73 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NT5E/CD73 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NT5E/CD73 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NT5E/CD73 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane.The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate
Background: Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane.The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate
Similar Products
Product Notes
The Rat NT5E/CD73 (Catalog #AAA9712706) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712706 ELISA Kit recognizes Rat NT5E/CD73. It is sometimes possible for the material contained within the vial of "5'-Nucleotidase/Cluster Of Differentiation 73 (NT5E/CD73), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
