Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DCN1-like protein 5 (DCUN1D5) Standard Curve.)
Rat DCN1-Like Protein 5 (DCUN1D5) ELISA Kit | DCUN1D5 elisa kit
Rat DCN1-Like Protein 5 (DCUN1D5) ELISA Kit
Gene Names
Dcun1d5; RGD1307008
Reactivity
Rat
Synonyms
DCN1-Like Protein 5 (DCUN1D5); Rat DCN1-Like Protein 5 (DCUN1D5) ELISA Kit; DCUN1D5; FLJ32431; FLJ37425; MGC2714; DCN1; defective in cullin neddylation 1; domain containing 5; DCUN1D5 elisa kit
Reactivity
Rat
Specificity
Rat DCN1-like protein 5 (DCUN1D5) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DCUN1D5. No significant cross-reactivity or interference between Rat DCUN1D5 and analogues was observed.
Sequence Length
237
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DCN1-like protein 5 (DCUN1D5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DCN1-like protein 5 (DCUN1D5) Standard Curve.)
Related Product Information for DCUN1D5 elisa kit
Description: This Rat DCN1-like protein 5 (DCUN1D5) ELISA Kit employs a two-site sandwich ELISA to quantitate DCUN1D5 in samples. An antibody specific for DCUN1D5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DCUN1D5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCUN1D5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCUN1D5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DCUN1D5 cDNA encoded a protein of 237 amino acids, which contained two domains associated with DNA repair and cell cycle regulation. Although no reported studies have clearly demonstrated DCUN1D5's role in cancer, one report suggested that DCUN1D5 expression may be useful as a biomarker for cervical cancer. Because DNA damage and repair pathways played critical roles in the development of LSCC, a smokingrelated cancer, and because DCUN1D5 was found to be up-regulated in our patients with LSCC who had a tobacco history, that DCUN1D5 had a vital function involved in DNA damage response and repair in the carcinogenesis of LSCC.
Background: DCUN1D5 cDNA encoded a protein of 237 amino acids, which contained two domains associated with DNA repair and cell cycle regulation. Although no reported studies have clearly demonstrated DCUN1D5's role in cancer, one report suggested that DCUN1D5 expression may be useful as a biomarker for cervical cancer. Because DNA damage and repair pathways played critical roles in the development of LSCC, a smokingrelated cancer, and because DCUN1D5 was found to be up-regulated in our patients with LSCC who had a tobacco history, that DCUN1D5 had a vital function involved in DNA damage response and repair in the carcinogenesis of LSCC.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DCN1-like protein 5
NCBI Official Synonym Full Names
defective in cullin neddylation 1 domain containing 5
NCBI Official Symbol
Dcun1d5
NCBI Official Synonym Symbols
RGD1307008
NCBI Protein Information
DCN1-like protein 5
UniProt Protein Name
DCN1-like protein 5
Protein Family
UniProt Gene Name
Dcun1d5
UniProt Entry Name
DCNL5_RAT
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Product Notes
The Rat DCUN1D5 dcun1d5 (Catalog #AAA9712461) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712461 ELISA Kit recognizes Rat DCUN1D5. It is sometimes possible for the material contained within the vial of "DCN1-Like Protein 5 (DCUN1D5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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