Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dehydroepiandrosterone (DHEA) Standard Curve.)
Rat Dehydroepiandrosterone (DHEA) ELISA Kit | DHEA elisa kit
Rat Dehydroepiandrosterone (DHEA) ELISA Kit
Reactivity
Rat
Synonyms
Dehydroepiandrosterone (DHEA); Rat Dehydroepiandrosterone (DHEA) ELISA Kit; DHEA elisa kit
Reactivity
Rat
Specificity
Rat Dehydroepiandrosterone (DHEA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DHEA. No significant cross-reactivity or interference between Rat DHEA and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dehydroepiandrosterone (DHEA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dehydroepiandrosterone (DHEA) Standard Curve.)
Related Product Information for DHEA elisa kit
Description: This Rat Dehydroepiandrosterone (DHEA) ELISA Kit employs a two-site sandwich ELISA to quantitate DHEA in samples. An antibody specific for DHEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHEA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHEA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHEA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Dehydroepiandrosterone (DHEA), also known as androstenolone or as prasterone (INN), is an endogenous steroid hormone. It is one of the most abundant circulating steroids in humans, in whom it is produced in the adrenal glands, the gonads, and the brain, where it functions as a metabolic intermediate in the biosynthesis of the androgen and estrogen sex steroids. However, DHEA also has a variety of potential biological effects in its own right, binding to an array of nuclear and cell surface receptors, and acting as a neurosteroid and neurotrophin. DHEA is also used orally as a medication and dietary supplement, to restore or increase DHEA and DHEA sulfate (DHEA-S) levels.
Background: Dehydroepiandrosterone (DHEA), also known as androstenolone or as prasterone (INN), is an endogenous steroid hormone. It is one of the most abundant circulating steroids in humans, in whom it is produced in the adrenal glands, the gonads, and the brain, where it functions as a metabolic intermediate in the biosynthesis of the androgen and estrogen sex steroids. However, DHEA also has a variety of potential biological effects in its own right, binding to an array of nuclear and cell surface receptors, and acting as a neurosteroid and neurotrophin. DHEA is also used orally as a medication and dietary supplement, to restore or increase DHEA and DHEA sulfate (DHEA-S) levels.
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Product Notes
The Rat DHEA (Catalog #AAA9712443) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712443 ELISA Kit recognizes Rat DHEA. It is sometimes possible for the material contained within the vial of "Dehydroepiandrosterone (DHEA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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