Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Neuroblastoma suppressor of tumorigenicity 1 (NBL1) Standard Curve.)
Rat Neuroblastoma Suppressor Of Tumorigenicity 1 (NBL1) ELISA Kit | NBL1 elisa kit
Rat Neuroblastoma Suppressor Of Tumorigenicity 1 (NBL1) ELISA Kit
Reactivity
Rat
Synonyms
Neuroblastoma Suppressor Of Tumorigenicity 1 (NBL1); Rat Neuroblastoma Suppressor Of Tumorigenicity 1 (NBL1) ELISA Kit; RP5-1056L3.4; D1S1733E; DAN; DAND1; MGC8972; NB; NO3; differential screening-selected gene aberrant in neuroblastoma; neuroblastoma candidate region; suppression of tumorigenicity 1; neuroblastoma sup; NBL1 elisa kit
Reactivity
Rat
Specificity
Rat Neuroblastoma suppressor of tumorigenicity 1 (NBL1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NBL1. No significant cross-reactivity or interference between Rat NBL1 and analogues was observed.
Sequence Length
178
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Neuroblastoma suppressor of tumorigenicity 1 (NBL1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Neuroblastoma suppressor of tumorigenicity 1 (NBL1) Standard Curve.)
Related Product Information for NBL1 elisa kit
Description: This Rat Neuroblastoma suppressor of tumorigenicity 1 (NBL1) ELISA Kit employs a two-site sandwich ELISA to quantitate NBL1 in samples. An antibody specific for NBL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NBL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NBL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NBL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NBL1 product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for NBL1. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist) is a Protein Coding gene. Diseases associated with NBL1 include Neuroblastoma and Regular Astigmatism. Among its related pathways are TGF-beta signaling pathway. GO annotations related to this gene include protein homodimerization activity and morphogen activity. An important paralog of this gene is MINOS1-NBL1.
Background: NBL1 product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for NBL1. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist) is a Protein Coding gene. Diseases associated with NBL1 include Neuroblastoma and Regular Astigmatism. Among its related pathways are TGF-beta signaling pathway. GO annotations related to this gene include protein homodimerization activity and morphogen activity. An important paralog of this gene is MINOS1-NBL1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
neuroblastoma suppressor of tumorigenicity 1
NCBI Official Synonym Full Names
NBL1, DAN family BMP antagonist
NCBI Official Symbol
Nbl1
NCBI Protein Information
neuroblastoma suppressor of tumorigenicity 1
UniProt Protein Name
Neuroblastoma suppressor of tumorigenicity 1
Protein Family
UniProt Gene Name
Nbl1
UniProt Synonym Gene Names
Dan
UniProt Entry Name
NBL1_RAT
NCBI Description
may be a tumor suppressor; found downregulated in transformed cells [RGD, Feb 2006]
Uniprot Description
NBL1: Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted; Tumor suppressor
Cellular Component: extracellular region
Molecular Function: morphogen activity; protein homodimerization activity
Biological Process: nervous system development; determination of dorsal identity; positive regulation of neuron differentiation; neurite morphogenesis; negative regulation of BMP signaling pathway
Research Articles on NBL1
Similar Products
Product Notes
The Rat NBL1 nbl1 (Catalog #AAA9712161) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712161 ELISA Kit recognizes Rat NBL1. It is sometimes possible for the material contained within the vial of "Neuroblastoma Suppressor Of Tumorigenicity 1 (NBL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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