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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Rat Cyclophilin A (CyPA) Standard Curve.)

Rat Cyclophilin A (CyPA) ELISA Kit | CyPA elisa kit

Rat Cyclophilin A (CyPA) ELISA Kit

Reactivity
Rat
Synonyms
Cyclophilin A (CyPA); Rat Cyclophilin A (CyPA) ELISA Kit; PPIA; CYPA; CYPH; MGC117158; MGC12404; MGC23397; PPIase A; T cell cyclophilin; cyclosporin A-binding protein; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A; rotamase A; CyPA elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
Rat Cyclophilin A (CyPA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PPIA. No significant cross-reactivity or interference between Rat PPIA and analogues was observed.
Sequence Length
164
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Cyclophilin A (CyPA) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Rat Cyclophilin A (CyPA) Standard Curve.)
Related Product Information for CyPA elisa kit
Description: This Rat Cyclophilin A (CyPA) ELISA Kit employs a two-site sandwich ELISA to quantitate PPIA in samples. An antibody specific for PPIA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPIA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPIA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPIA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Cyclophilin A, also called Peptidyl-prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis- and trans- proline residues critical for proper folding of proteins. Cyclophilin is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity. The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity and prevent incorporation of cyclophilin into viral particles. The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin, making it a useful inhibitor for studying calcineurin activity.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase A
NCBI Official Symbol
PPIA
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase A
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase A
Protein Family
UniProt Gene Name
PPIA
UniProt Synonym Gene Names
PPIase A
UniProt Entry Name
PPIA_RABIT

Uniprot Description

Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Catalytic activity: Peptidylproline (omega=180) = peptidylproline (omega=0).

Enzyme regulation: Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase

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Product Notes

The Rat CyPA ppia (Catalog #AAA9712097) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712097 ELISA Kit recognizes Rat CyPA. It is sometimes possible for the material contained within the vial of "Cyclophilin A (CyPA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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