Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sonic hedgehog protein (SHH) Standard Curve.)
Rat Sonic Hedgehog Protein (SHH) ELISA Kit | SHH elisa kit
Rat Sonic Hedgehog Protein (SHH) ELISA Kit
Gene Names
Shh; ShhNC
Reactivity
Rat
Synonyms
Sonic Hedgehog Protein (SHH); Rat Sonic Hedgehog Protein (SHH) ELISA Kit; SHH; HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog; SHH elisa kit
Reactivity
Rat
Specificity
Rat Sonic hedgehog protein (SHH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SHH. No significant cross-reactivity or interference between Rat SHH and analogues was observed.
Sequence Length
437
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sonic hedgehog protein (SHH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Sonic hedgehog protein (SHH) Standard Curve.)
Related Product Information for SHH elisa kit
Description: This Rat Sonic hedgehog protein (SHH) ELISA Kit employs a two-site sandwich ELISA to quantitate SHH in samples. An antibody specific for SHH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SHH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SHH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SHH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sonic hedgehog (Shh) is a member of a small family of secreted proteins that are essential for development in both vertebrates and invertebrates. In mammals, there are three related hedgehog genes (sonic; desert; Indian) which share approximately 60 % amino acid (aa) identity. Human Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing, glycosylated, 34 kDa, 239 aa C-terminal fragment.The 20 kDa Shh-N fragment is the core of the active hedgehog molecule. It begins with a Cys and ends with a Gly, and both amino acids are sites of lipid modification.
Background: Sonic hedgehog (Shh) is a member of a small family of secreted proteins that are essential for development in both vertebrates and invertebrates. In mammals, there are three related hedgehog genes (sonic; desert; Indian) which share approximately 60 % amino acid (aa) identity. Human Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing, glycosylated, 34 kDa, 239 aa C-terminal fragment.The 20 kDa Shh-N fragment is the core of the active hedgehog molecule. It begins with a Cys and ends with a Gly, and both amino acids are sites of lipid modification.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sonic hedgehog protein
NCBI Official Synonym Full Names
sonic hedgehog signaling molecule
NCBI Official Symbol
Shh
NCBI Official Synonym Symbols
ShhNC
NCBI Protein Information
sonic hedgehog protein
UniProt Protein Name
Sonic hedgehog protein
Protein Family
UniProt Gene Name
Shh
UniProt Synonym Gene Names
Vhh-1; SHH
NCBI Description
plays a role in neurogenesis; regulates proliferation of adult hippocampal neural stem cells [RGD, Feb 2006]
Uniprot Description
Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO ().
Research Articles on SHH
Similar Products
Product Notes
The Rat SHH shh (Catalog #AAA9711883) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711883 ELISA Kit recognizes Rat SHH. It is sometimes possible for the material contained within the vial of "Sonic Hedgehog Protein (SHH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
