Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transmembrane protease serine 9 (TMPRSS9) Standard Curve.)
Rat Transmembrane Protease Serine 9 (TMPRSS9) ELISA Kit | TMPRSS9 elisa kit
Rat Transmembrane Protease Serine 9 (TMPRSS9) ELISA Kit
Reactivity
Rat
Synonyms
Transmembrane Protease Serine 9 (TMPRSS9); Rat Transmembrane Protease Serine 9 (TMPRSS9) ELISA Kit; TMPRSS9; FLJ16193; polyserase 1; transmembrane serine protease 9; TMPRSS9 elisa kit
Reactivity
Rat
Specificity
Rat Transmembrane protease serine 9 (TMPRSS9) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TMPRSS9. No significant cross-reactivity or interference between Rat TMPRSS9 and analogues was observed.
Sequence Length
1061
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transmembrane protease serine 9 (TMPRSS9) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transmembrane protease serine 9 (TMPRSS9) Standard Curve.)
Related Product Information for TMPRSS9 elisa kit
Description: This Rat Transmembrane protease serine 9 (TMPRSS9) ELISA Kit employs a two-site sandwich ELISA to quantitate TMPRSS9 in samples. An antibody specific for TMPRSS9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMPRSS9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMPRSS9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPRSS9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TMPRSS9 contains a predicted type II transmembrane segment, an LDLR domain, and 3 protease domains, which they called serase-1, -2, and -3. The C-terminal serase-3 domain is predicted to be catalytically inactive. TMPRSS9 shares approximately 80% amino acid identity with both mouse and rat Tmprss9, and the protease domains share 40% to 48% amino acid identity with matriptase (ST14) and matriptase-2 (TMPRSS6). Northern blot analysis detected a 5.4-kb transcript in all adult tissues examined, fetal kidney, liver, lung, and brain, and in several tumor cell lines. Minor transcripts of 3.8 and 2.4 kb were detected in adult skeletal muscle, liver, placenta, and heart. Immunofluorescence microscopy localized TMPRSS9 to the plasma membrane in COS-7 cells.
Background: TMPRSS9 contains a predicted type II transmembrane segment, an LDLR domain, and 3 protease domains, which they called serase-1, -2, and -3. The C-terminal serase-3 domain is predicted to be catalytically inactive. TMPRSS9 shares approximately 80% amino acid identity with both mouse and rat Tmprss9, and the protease domains share 40% to 48% amino acid identity with matriptase (ST14) and matriptase-2 (TMPRSS6). Northern blot analysis detected a 5.4-kb transcript in all adult tissues examined, fetal kidney, liver, lung, and brain, and in several tumor cell lines. Minor transcripts of 3.8 and 2.4 kb were detected in adult skeletal muscle, liver, placenta, and heart. Immunofluorescence microscopy localized TMPRSS9 to the plasma membrane in COS-7 cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transmembrane protease serine 9
NCBI Official Synonym Full Names
transmembrane serine protease 9
NCBI Official Symbol
Tmprss9
NCBI Protein Information
transmembrane protease serine 9
UniProt Protein Name
Transmembrane protease serine 9
Protein Family
UniProt Gene Name
Tmprss9
UniProt Synonym Gene Names
Polyserase-1
UniProt Entry Name
TMPS9_RAT
Research Articles on TMPRSS9
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Product Notes
The Rat TMPRSS9 tmprss9 (Catalog #AAA9711687) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711687 ELISA Kit recognizes Rat TMPRSS9. It is sometimes possible for the material contained within the vial of "Transmembrane Protease Serine 9 (TMPRSS9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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