Rabbit anti-Human Caspase 12 Polyclonal Antibody | anti-CASP12 antibody

Caspase 12 Polyclonal Antibody

Cat. Num. AAA9701581

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Caspase 12; Polyclonal Antibody; Caspase 12 Polyclonal Antibody; Rabbit Anti-Caspase12 Polyclonal Antibody; Inactive caspase-12; CASP-12; anti-CASP12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Caspase12 Polyclonal Antibody detects endogenous levels of Caspase12 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 341

• Applicable Applications for anti-CASP12 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:300; ELISA: 1:40000
• Immunogen: Synthesized peptide derived from the internal region of human Caspase12
• Preparation and Storage: Stable for one year at -20 degree C from date of shipment.; For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig. Western Blot analysis of various cells using Caspase12 Polyclonal Antibody diluted at 1:500.)

Related Product Information for anti-CASP12 antibody

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. CASP12 is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of CASP12 mediates apoptosis in response to endoplasmic reticulum stress. However, in humans CASP12 contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for CASP12.

NCBI and Uniprot Product Information

• NCBI GI #: 395398427
• UniProt Accession #: Q6UXS9
• NCBI Official Full Name: Inactive caspase-12
• UniProt Protein Name: Inactive caspase-12
• Protein Family: Inactive caspase
• UniProt Gene Name: CASP12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_HUMAN

Uniprot Description

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.-

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: cytoplasm

Molecular Function: cysteine-type endopeptidase activity

Biological Process: regulation of apoptosis; unfolded protein response; regulation of inflammatory response; proteolysis