Rabbit CASP12 Polyclonal Antibody | anti-CASP12 antibody

CASP12 Polyclonal Antibody

Cat. Num. AAA2529292

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: CASP12; Polyclonal Antibody; CASP12 Polyclonal Antibody; CASP-12; CASP12P1; anti-CASP12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1.5mg/mL (varies by lot)
• Sequence Length: 420

• Applicable Applications for anti-CASP12 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide of human CASP12
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of NIH/3T3 cell using CASP12 Polyclonal Antibody at dilution of 1:700)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer using CASP12 Polyclonal Antibody at dilution of 1:50)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human lung cancer using CASP12 Polyclonal Antibody at dilution of 1:50)

Related Product Information for anti-CASP12 antibody

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene.

Product Categories/Family for anti-CASP12 antibody

Cancer; Cell biology

NCBI and Uniprot Product Information

• NCBI GI #: 18426852
• NCBI GeneID: 156117
• NCBI Accession #: NP_569106.1
• NCBI GenBank Nucleotide #: NM_130422.1
• Molecular Weight: 47,837 Da
• NCBI Official Full Name: caspase-12
• NCBI Official Synonym Full Names: caspase 12
• NCBI Official Symbol: Casp12
• NCBI Protein Information: caspase-12; CASP-12
• UniProt Protein Name: Caspase-12
• Protein Family: Inactive caspase
• UniProt Gene Name: Casp12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_RAT

NCBI Description

cysteine-aspartic acid protease (caspase); involved with the terminal stage of apoptosis [RGD, Feb 2006]

Uniprot Description

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.-; Protease

Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; intracellular; cytosol; nucleus

Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: response to nicotine; response to drug; response to inorganic substance; response to anoxia; response to iron(III) ion; positive regulation of apoptosis; apoptosis; unfolded protein response; positive regulation of caspase activity; response to organic nitrogen; proteolysis; regulation of apoptosis; protein autoprocessing; response to ethanol; regulation of inflammatory response; response to cold; aging