Rabbit GCN1 Polyclonal Antibody | anti-GCN1 antibody
GCN1 Antibody
Gene Names
GCN1L1; GCN1; GCN1L; PRIC295
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
GCN1; Polyclonal Antibody; GCN1 Antibody; eIF-2-alpha kinase activator GCN1; GCN1 (general control of amino acid synthesis yeast homolog) like; GCN1 (general control of amino acid synthesis 1 yeast) like 1; GCN1 eIF-2-alpha kinase activator homolog; GCN1 general control of amino acid synthesis 1 like 1; GCN1 general control of amino acid synthesis 1 like 1 (yeast); GCN1 like protein 1; GCN1-like protein 1; GCN1_HUMAN; GCN1L; General control of amino-acid synthesis 1-like protein 1; HsGCN1; Translational activator gcn1; anti-GCN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
GCN1 Antibody detects endogenous levels of GCN1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GCN1 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human GCN1.
Conjugation
Unconjugated
Similarity
Belongs to the GCN1 family.
Subunit Structure
Interacts with EIF2AK4/GCN2; this interaction stimulates the EIF2AK4/GCN2 kinase activity and is impaired by IMPACT upon a variety of stress conditions, such as amino acid depletion, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Interacts with IMPACT; this prevents the interaction of GCN1 with EIF2AK4/GCN2 and inhibits EIF2AK4/GCN2 kinase activity.
Tissue Specificity
Ubiquitously expressed. Expressed in skeletal muscules, ovary and testis.
Function
Acts as a positive activator of the EIF2AK4/GCN2 protein kinase activity in response to amino acid starvation. Forms a complex with EIF2AK4/GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of EIF2AK4/GCN2, and hence stimulating EIF2AK4/GCN2 kinase activity. Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator ATF4, by promoting the EIF2AK4/GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52', and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion.
Note
Associates with ribosomes in undifferentiated neuroblastoma cells and increases after neuronal differentiation.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
292,758 Da
NCBI Official Full Name
translational activator GCN1
NCBI Official Synonym Full Names
GCN1 general control of amino-acid synthesis 1-like 1 (yeast)
NCBI Official Symbol
GCN1L1
NCBI Official Synonym Symbols
GCN1; GCN1L; PRIC295
NCBI Protein Information
translational activator GCN1; hsGCN1; GCN1-like protein 1; GCN1 (general control of amino-acid synthesis 1, yeast)-like 1; peroxisome proliferator activated receptor interacting complex protein
UniProt Protein Name
Translational activator GCN1
Protein Family
UniProt Gene Name
GCN1L1
UniProt Synonym Gene Names
KIAA0219; HsGCN1
UniProt Entry Name
GCN1L_HUMAN
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Product Notes
The GCN1 gcn1l1 (Catalog #AAA9631229) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GCN1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GCN1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the GCN1 gcn1l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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