Rabbit RTEL1 Polyclonal Antibody | anti-RTEL1 antibody

RTEL1 Antibody

Cat. Num. AAA9631206

• Gene Names: RTEL1; NHL; RTEL; DKCA4; DKCB5; C20orf41
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RTEL1; Polyclonal Antibody; RTEL1 Antibody; bK3184A7.3; C20orf41; Chromosome 20 open reading frame 41; DKCA4; DKCB5; DKFZP434C013; KIAA1088; NHL; Novel helicase like; PFBMFT3; Regulator of telomere elongation helicase 1; Regulator of telomere length; RP4-583P15.2; RTEL; anti-RTEL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RTEL1 Antibody detects endogenous levels of RTEL1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RTEL1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human RTEL1.
• Conjugation: Unconjugated
• Similarity: The PIP-box (PCNA interacting peptide) motif mediates the interaction with PCNA and localization to replication foci. Belongs to the helicase family. RAD3/XPD subfamily.
• Subunit Structure: Interacts with TERF1. Interacts (via PIP-box) with PCNA; the interaction is direct and essential for suppressing telomere fragility. Interacts with MMS19; the interaction mediates the association of RTEL1 with the cytosolic iron-sulfur protein assembly (CIA) complex.
• Function: ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates. Also disassembles T loops and prevents telomere fragility by counteracting telomeric G4-DNA structures, which together ensure the dynamics and stability of the telomere.
• Note: Colocalizes with PCNA within the replication foci in S-phase cells.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RTEL1 antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 7706541
• NCBI GeneID: 51750
• NCBI Accession #: NP_057518.1
• NCBI GenBank Nucleotide #: NM_016434.3
• UniProt Accession #: Q9NZ71; Q5JTV3; Q5JTV4; Q9BW37; Q9H402; A2A397; A2A398; B4DRM5; B4DYM3; B4E3N6; E1P5J4; E1P5J5
• Molecular Weight: 108,457 Da
• NCBI Official Full Name: regulator of telomere elongation helicase 1 isoform 1
• NCBI Official Synonym Full Names: regulator of telomere elongation helicase 1
• NCBI Official Symbol: RTEL1
• NCBI Official Synonym Symbols: NHL; RTEL; DKCA4; DKCB5; C20orf41
• NCBI Protein Information: regulator of telomere elongation helicase 1; RTEL1; regulator of telomere length
• UniProt Protein Name: Regulator of telomere elongation helicase 1
• Protein Family: Regulator of telomere elongation helicase
• UniProt Gene Name: RTEL1
• UniProt Entry Name: RTEL1_HUMAN

NCBI Description

This gene encodes a DNA helicase which functions in the stability, protection and elongation of telomeres and interacts with proteins in the shelterin complex known to protect telomeres during DNA replication. Mutations in this gene have been associated with dyskeratosis congenita and Hoyerall-Hreidarsson syndrome. Read-through transcription of this gene into the neighboring downstream gene, which encodes tumor necrosis factor receptor superfamily, member 6b, generates a non-coding transcript. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013]

Uniprot Description

RTEL1: ATP-dependent DNA helicase required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by promoting the disassembly of D loop recombination intermediates. Also required to regulate telomere length; probably due to its anti-recombinase function. Belongs to the helicase family. RAD3/XPD subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; DNA repair, damage; Helicase

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; 4 iron, 4 sulfur cluster binding; metal ion binding; ATP binding

Biological Process: DNA repair; telomere maintenance; DNA duplex unwinding

Disease: Dyskeratosis Congenita, Autosomal Recessive, 5; Pulmonary Fibrosis And/or Bone Marrow Failure, Telomere-related, 3