Rabbit anti-Human LILRB3 Polyclonal Antibody | anti-LILRB3 antibody

LILRB3 Antibody

Cat. Num. AAA9631131

• Gene Names: LILRB3; HL9; ILT5; LIR3; PIRB; CD85A; ILT-5; LIR-3; PIR-B; LILRA6
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: LILRB3; Polyclonal Antibody; LILRB3 Antibody; CD85 antigen like family member A; CD85a; CD85a antigen; HL9; IL T5; ILT5; Immunoglobulin like transcript 5; Leukocyte immunoglobulin like receptor 3; Leukocyte immunoglobulin like receptor subfamily B (with TM and ITIM domains) member 3; Leukocyte immunoglobulin like receptor subfamily B member 3; LIR 3; LIR3; MGC138403; Monocyte inhibitory receptor HL9; PIRB; anti-LILRB3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: LILRB3 Antibody detects endogenous levels of LILRB3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LILRB3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human LILRB3.
• Conjugation: Unconjugated
• Similarity: Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, including PTPN6/SHP-1, resulting in the dephosphorylation of the downstream protein kinases SYK and BTK.
• Post Translational Modifications: Phosphorylated on tyrosine residues by LYN. Phosphorylation at Tyr-595 and Tyr-625 is important for interaction with PTPN6/SHP-1 and PTPN11/SHP-2.
• Subunit Structure: Interacts with LYN, PTPN6/SHP-1 and PTPN11/SHP-2.
• Tissue Specificity: Detected in monocytes and B-cells.
• Function: May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-LILRB3 antibody

Cell membrane; Single-pass type I membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 574270893
• NCBI GeneID: 11025
• NCBI Accession #: NP_001074919.2
• NCBI GenBank Nucleotide #: NM_001081450.2
• UniProt Accession #: O75022; O15471; Q86U49; C9J1P3; C9JIP1
• Molecular Weight: 69,514 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 3 isoform 1
• NCBI Official Synonym Full Names: leukocyte immunoglobulin like receptor B3
• NCBI Official Symbol: LILRB3
• NCBI Official Synonym Symbols: HL9; ILT5; LIR3; PIRB; CD85A; ILT-5; LIR-3; PIR-B; LILRA6
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 3
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 3
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRB3
• UniProt Synonym Gene Names: ILT5; LIR3; LIR-3; Leukocyte immunoglobulin-like receptor 3; ILT-5
• UniProt Entry Name: LIRB3_HUMAN

NCBI Description

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LILRB3: May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B- cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: integral to plasma membrane

Molecular Function: protein binding; receptor activity; transmembrane receptor activity

Biological Process: adaptive immune response; cell surface receptor linked signal transduction; defense response; negative regulation of osteoclast differentiation