Rabbit anti-Human POLK Polyclonal Antibody | anti-POLK antibody

POLK Antibody

Cat. Num. AAA9631044

• Gene Names: POLK; DINP; POLQ; DINB1
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: POLK; Polyclonal Antibody; POLK Antibody; DinB homolog 1 (E. coli); DINB protein; DINB1; DINP; DNA damage inducible protein b; DNA polymerase kappa; Polk; POLK_HUMAN; POLQ; Polymerase (DNA directed) kappa; polymerase; DNA; kappa; anti-POLK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: POLK Antibody detects endogenous levels of POLK.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-POLK antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human POLK.
• Conjugation: Unconjugated
• Similarity: The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. Belongs to the DNA polymerase type-Y family.
• Subunit Structure: Interacts with REV1 (By similarity). Interacts with PCNA.
• Tissue Specificity: Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
• Function: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
• Note: Detected throughout the nucleus and at replication foci (PubMed:12414988). Recruited to DNA damage sites in response to ultraviolet irradiation: N6-methyladenosine (m6A)-containing mRNAs accumulate in the vicinity of DNA damage sites and their presence is required to recruit POLK (PubMed:28297716).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-POLK antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 7705344
• NCBI GeneID: 51426
• NCBI Accession #: NP_057302.1
• NCBI GenBank Nucleotide #: NM_016218.2
• UniProt Accession #: Q9UBT6; Q5Q9G5; Q5Q9G6; Q5Q9G7; Q5Q9G8; Q86VJ8; Q8IZY0; Q8IZY1; Q8NB30; Q96L01; Q96Q86; B2RBD2
• Molecular Weight: 88,342 Da
• NCBI Official Full Name: DNA polymerase kappa
• NCBI Official Synonym Full Names: polymerase (DNA directed) kappa
• NCBI Official Symbol: POLK
• NCBI Official Synonym Symbols: DINP; POLQ; DINB1
• NCBI Protein Information: DNA polymerase kappa
• UniProt Protein Name: DNA polymerase kappa
• UniProt Gene Name: POLK
• UniProt Synonym Gene Names: DINB1; DINP
• UniProt Entry Name: POLK_HUMAN

NCBI Description

External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]).[supplied by OMIM, Jan 2010]

Uniprot Description

POLK: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Belongs to the DNA polymerase type-Y family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Transferase; EC 2.7.7.7; DNA replication

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: nucleoplasm

Molecular Function: metal ion binding; damaged DNA binding; DNA-directed DNA polymerase activity

Biological Process: nucleotide-excision repair, DNA gap filling; DNA repair; DNA replication