Rabbit anti-Human ASAH2 Polyclonal Antibody | anti-ASAH2 antibody

ASAH2 Antibody

Cat. Num. AAA9630955

• Gene Names: ASAH2; HNAC1; BCDase; LCDase; NCDase; N-CDase
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ASAH2; Polyclonal Antibody; ASAH2 Antibody; Acylsphingosine deacylase 2; AI585898; ASAH2_HUMAN; BCDase; hCD; HNAC1; LCDase; MGC129777; mitochondrial ceramidase; N acylsphingosine amidohydrolase (non lysosomal ceramidase) 2; N acylsphingosine amidohydrolase 2; N CDase; N-acylsphingosine amidohydrolase 2; N-CDase; NCDase; Neutral ceramidase soluble form; Non lysosomal ceramidase; Non-lysosomal ceramidase; anti-ASAH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ASAH2 Antibody detects endogenous levels of ASAH2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ASAH2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human ASAH2.
• Conjugation: Unconjugated
• Similarity: Belongs to the neutral ceramidase family.
• Post Translational Modifications: Proteolytic cleavage of the N-terminus removes the signal-anchor and produces a soluble form of the protein.N-glycosylated. Required for enzyme activity.O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.Phosphorylated. May prevent ubiquitination and subsequent degradation.Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxide.
• Tissue Specificity: Primarily expressed in intestine. Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart. The ubiquitous expression observed for ASAH2 might be an experimental artifact due to the paralog ASAH2B.
• Function: Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells. Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (By similarity). This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (By similarity).
• Note: Enriched in exosomes upon stimulation by cytokine (PubMed:24798654). Enriched in caveolae and lipid rafts (By similarity). The localization to the mitochondrion could not be confirmed (PubMed:15845354).Secreted.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ASAH2 antibody

Cell membrane; Single-pass type II membrane protein; Membrane raft; Membrane; Caveola; Golgi apparatus membrane; Mitochondrion; Secreted; Extracellular exosome; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 221218981
• NCBI GeneID: 56624
• NCBI Accession #: NP_063946
• NCBI GenBank Nucleotide #: NM_019893.2
• UniProt Accession #: Q9NR71; Q3KNU1; Q5SNT7; Q5SZP6; Q5SZP7; Q5T1D5; Q71ME6
• Molecular Weight: 81,718 Da
• NCBI Official Full Name: neutral ceramidase isoform a
• NCBI Official Synonym Full Names: N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
• NCBI Official Symbol: ASAH2
• NCBI Official Synonym Symbols: HNAC1; BCDase; LCDase; NCDase; N-CDase
• NCBI Protein Information: neutral ceramidase; mitochondrial ceramidase; non-lysosomal ceramidase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2
• UniProt Protein Name: Neutral ceramidase
• Protein Family: Neutral ceramidase
• UniProt Gene Name: ASAH2
• UniProt Synonym Gene Names: HNAC1; N-CDase; NCDase; hCD
• UniProt Entry Name: ASAH2_HUMAN

NCBI Description

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]

Uniprot Description

ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Hydrolase; EC 3.5.1.23; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: mitochondrion; plasma membrane; integral to membrane

Molecular Function: ceramidase activity

Biological Process: sphingolipid metabolic process; apoptosis; glycosphingolipid metabolic process; ceramide metabolic process; signal transduction