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Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using OCT3 antibody.)

OCT3 Antibody | anti-OCT3 antibody

OCT3 Antibody

Gene Names
POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
OCT3; OCT3 Antibody; OCT4; OTF3; OTF4; OCTAMER-BINDING TRANSCRIPTION FACTOR 3; OCTAMER-BINDING TRANSCRIPTION FACTOR 4; anti-OCT3 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
OCT3 antibody detects endogenous levels of total OCT3 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
265
Applicable Applications for anti-OCT3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from human OCT3 antibody.
Preparation and Storage
Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using OCT3 antibody.)

Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using OCT3 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using OCT3 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells, using OCT3 antibody.)
References
Y Saijoh, Genes Cells, Feb 1996; 1: 239-252. Christopher L. Shaffer, Drug Metab. Dispos., Apr 2007; 10.1124. Brian Boer, Nucleic Acids Res., Mar 2007; 35: 1773-1786. E Pikarsky, Mol. Cell. Biol., Feb 1994; 14: 1026-1038.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,085 Da
NCBI Official Full Name
POU domain, class 5, transcription factor 1 isoform 3
NCBI Official Synonym Full Names
POU class 5 homeobox 1
NCBI Official Symbol
POU5F1
NCBI Official Synonym Symbols
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
NCBI Protein Information
POU domain, class 5, transcription factor 1
UniProt Protein Name
POU domain, class 5, transcription factor 1
UniProt Gene Name
POU5F1
UniProt Synonym Gene Names
OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3

NCBI Description

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Uniprot Description

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Research Articles on OCT3

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Product Notes

The OCT3 pou5f1 (Catalog #AAA853105) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The OCT3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's OCT3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000. Researchers should empirically determine the suitability of the OCT3 pou5f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OCT3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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