Rabbit anti-Human, Mouse LIMA1 Polyclonal Antibody | anti-LIMA1 antibody

LIMA1 Antibody

Cat. Num. AAA9630922

• Gene Names: LIMA1; EPLIN; SREBP3
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: LIMA1; Polyclonal Antibody; LIMA1 Antibody; 1110021C24Rik; Epithelial protein lost in neoplasm; Epithelial protein lost in neoplasm beta; EPLIN; FLJ38853; LIM domain and actin binding 1; LIM domain and actin binding protein 1; LIM domain and actin-binding protein 1; LIMA1_HUMAN; MGC131726; SREBP3; Sterol regulatory element binding protein 3; anti-LIMA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: LIMA1 Antibody detects endogenous levels of LIMA1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LIMA1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human LIMA1.
• Conjugation: Unconjugated
• Similarity: Contains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than the N-terminus.
• Post Translational Modifications: Phosphorylation of the C-terminal region by MAPK1/MAPK3 reduces its association with F-actin and contributes to actin filament reorganization and enhances cell motility.
• Subunit Structure: Interacts with NPCL1; bridges NPC1L1 with MYO5B. Interacts with MYO5B; bridges NPC1L1 with MYO5B. Interacts with PXN; this complex stabilizes actin dynamics. Interacts with F-actin and G-actin.
• Tissue Specificity: Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium.
• Function: Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (By similarity).
• Note: Expressed in the brush border membrane of the small intestine and colocalizes with NPC1L1 and MYO5B (PubMed:29880681). Colocalizes with PXN at focal adhesions in mesangial cells (PubMed:24694988). Colocalizes with actin stress fibers in quiescent cells. PDGF stimulation induced disassembly of stress fibers and formation of peripheral and dorsal ruffles, where LIMA1 is relocalized (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-LIMA1 antibody

Cytoplasm; Cell junction; Focal adhesion; Cytoskeleton; Stress fiber; Cell membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 165905589
• NCBI GeneID: 51474
• NCBI Accession #: NP_001107018
• NCBI GenBank Nucleotide #: NM_001113546.1
• UniProt Accession #: Q9UHB6; Q2TAN7; Q59FE8; Q9BVF2; Q9H8J1; Q9HBN5; Q9NX96; Q9NXC3; Q9NXU6; Q9P0H8; Q9UHB5; B2RB09
• Molecular Weight: Predicted: 50; 66; 84 kDa; ; Observed: 65 kDa
• NCBI Official Full Name: LIM domain and actin-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: LIM domain and actin binding 1
• NCBI Official Symbol: LIMA1
• NCBI Official Synonym Symbols: EPLIN; SREBP3
• NCBI Protein Information: LIM domain and actin-binding protein 1; 1110021C24Rik; epithelial protein lost in neoplasm beta; sterol regulatory element binding protein 3
• UniProt Protein Name: LIM domain and actin-binding protein 1
• Protein Family: LIM domain and actin-binding protein
• UniProt Gene Name: LIMA1
• UniProt Synonym Gene Names: EPLIN; SREBP3
• UniProt Entry Name: LIMA1_HUMAN

NCBI Description

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]

Uniprot Description

eplin: Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: focal adhesion; cytoplasm; plasma membrane; stress fiber; cleavage furrow; actin cytoskeleton

Molecular Function: actin monomer binding; actin filament binding; protein binding; zinc ion binding

Biological Process: actin filament bundle formation; ruffle organization and biogenesis; negative regulation of actin filament depolymerization