Rabbit A1CF Polyclonal Antibody | anti-A1CF antibody

A1CF Antibody

Cat. Num. AAA9630798

• Gene Names: A1CF; ACF; ASP; ACF64; ACF65; APOBEC1CF; RP11-564C4.2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: A1CF; Polyclonal Antibody; A1CF Antibody; A1CF_HUMAN; ACF; ACF64; ACF65; Apo B RNA editing protein; Apobec 1 complementation factor; Apobec 1 complementation factor (ACF) (ASP); APOBEC 1 stimulating protein; APOBEC1 complementation factor; APOBEC1 stimulating protein; APOBEC1-stimulating protein; APOBEC1CF; ASP; MGC163391; RP11-564C4.2; anti-A1CF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: A1CF Antibody detects endogenous levels of A1CF.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-A1CF antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human A1CF.
• Conjugation: Unconjugated
• Similarity: The RRM domains are necessary but not sufficient for binding to APOB mRNA. Additional residues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementing APOBEC1 activity.
• Subunit Structure: Part of the apolipoprotein B mRNA editing complex with APOBEC1. Interacts with TNPO2; TNPO2 may be responsible for transport of A1CF into the nucleus. Interacts with SYNCRIP. Interacts with CELF2/CUGBP2 (By similarity).
• Tissue Specificity: Widely expressed with highest levels in brain, liver, pancreas, colon and spleen.
• Function: Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
• Note: Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-A1CF antibody

Nucleus; Endoplasmic reticulum; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 219521544
• NCBI GeneID: 29974
• UniProt Accession #: Q9NQ94; Q5SZQ0; Q9NQ93; Q9NQX8; Q9NQX9; Q9NXC9; Q9NZD3; A1L4F2; A8K7G7; B7ZM14
• Molecular Weight: 65,202 Da
• NCBI Official Full Name: A1CF protein
• NCBI Official Synonym Full Names: APOBEC1 complementation factor
• NCBI Official Symbol: A1CF
• NCBI Official Synonym Symbols: ACF; ASP; ACF64; ACF65; APOBEC1CF; RP11-564C4.2
• NCBI Protein Information: APOBEC1 complementation factor; apo-B RNA editing protein; APOBEC-1 stimulating protein; apobec-1 complementation factor (ACF) (ASP)
• UniProt Protein Name: APOBEC1 complementation factor
• Protein Family: APOBEC1 complementation factor
• UniProt Gene Name: A1CF
• UniProt Synonym Gene Names: ACF; ASP
• UniProt Entry Name: A1CF_HUMAN

NCBI Description

Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

ACF: Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; RNA-binding; Hydrolase

Chromosomal Location of Human Ortholog: 10q11.23

Cellular Component: nucleoplasm; endoplasmic reticulum; cytoplasm; apolipoprotein B mRNA editing enzyme complex

Molecular Function: protein binding; single-stranded RNA binding; RNA binding; double-stranded RNA binding; nucleotide binding

Biological Process: protein stabilization; cytidine to uridine editing; mRNA modification; gene expression; mRNA processing