Rabbit anti-Human, Mouse UNKL Polyclonal Antibody | anti-UNKL antibody
UNKL Antibody
Gene Names
UNKL; ZC3H5L; C16orf28; ZC3HDC5L
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
UNKL; Polyclonal Antibody; UNKL Antibody; anti-UNKL antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
UNKL Antibody detects endogenous levels of UNKL.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-UNKL antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human UNKL.
Conjugation
Unconjugated
Similarity
Although this protein contains a RING domain, intrinsic E3 ligase activity has not been proven. Belongs to the unkempt family.
Post Translational Modifications
Isoform 4 is ubiquitinated in the C-terminal. Ubiquitination is enhanced by activated RAC1. The presence of the RING finger domain is not essential for ubiquitination to occur.
Subunit Structure
Isoform 4 (C-terminal) interacts with the GTP-bound form of RAC1. Isoform 4 (C-terminal) interacts with SMARCD2/BAF60b.
Function
May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
Note
Isoform 4 is primarily localized in the cytoplasm but has the ability to shuttle between the nucleus and the cytoplasm.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
680
NCBI Official Full Name
Putative E3 ubiquitin-protein ligase UNKL
NCBI Official Synonym Full Names
unkempt family zinc finger-like
NCBI Official Symbol
UNKL
NCBI Official Synonym Symbols
ZC3H5L; C16orf28; ZC3HDC5L
NCBI Protein Information
putative E3 ubiquitin-protein ligase UNKL; unkempt homolog-like; RING finger protein unkempt-like; zinc finger CCCH domain-containing protein 5-like
UniProt Protein Name
Putative E3 ubiquitin-protein ligase UNKL
Protein Family
UniProt Gene Name
UNKL
UniProt Synonym Gene Names
C16orf28; ZC3H5L; ZC3HDC5L
UniProt Entry Name
UNKL_HUMAN
NCBI Description
This gene encodes a RING finger protein that may function in Rac signaling. It can bind to Brg/Brm-associated factor 60b and can promote its ubiquitination. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Uniprot Description
UNKL: May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven. Belongs to the unkempt family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; zinc ion binding; ligase activity
Biological Process: protein ubiquitination
Research Articles on UNKL
Similar Products
Product Notes
The UNKL unkl (Catalog #AAA9630648) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UNKL Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UNKL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the UNKL unkl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UNKL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
